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Literature summary extracted from

  • Preiss, J.; Wood, E.
    Sugar nucleotide reactions in Arthrobacter. I. Guanosine diphosphate mannose pyrophosphorylase: purification and properties (1964), J. Biol. Chem., 239, 3119-3126.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.13 0.035
-
 alpha-D-mannose 1-phosphate pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 0.052
-
 GDP-mannose pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 0.09
-
 diphosphate pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 0.12
-
 dGDP-mannose pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 0.13
-
 GTP pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 0.28
-
 ITP pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 1.9
-
 Mg2+ pH 8.2, 37°C Arthrobacter sp.
2.7.7.13 3.3
-
 IDP-mannose pH 8.2, 37°C Arthrobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Arthrobacter sp.
-
 GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate Arthrobacter sp. NRRL B1973
-
 GDPmannose + diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.13 Arthrobacter sp.
-
 NRRL B1973
-
2.7.7.13 Arthrobacter sp. NRRL B1973
-
 NRRL B1973
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.13 protamine sulfate, Sephadex G-25, DEAE-cellulose Arthrobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.13 2.4
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.13 dGTP + alpha-D-mannose 1-phosphate 8% of activity with GDP-mannose Arthrobacter sp. dGDPmannose + diphosphate
-
 r
2.7.7.13 dGTP + alpha-D-mannose 1-phosphate 8% of activity with GDP-mannose Arthrobacter sp. NRRL B1973 dGDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Arthrobacter sp. GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate equilibrium constant: 2.5 Arthrobacter sp. GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Arthrobacter sp. NRRL B1973 GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate equilibrium constant: 2.5 Arthrobacter sp. NRRL B1973 GDPmannose + diphosphate
-
 r
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Arthrobacter sp. diphosphate + GDP-mannose
-
 ?
2.7.7.13 GTP + alpha-D-mannose 1-phosphate
-
 Arthrobacter sp. NRRL B1973 diphosphate + GDP-mannose
-
 ?
2.7.7.13 ITP + alpha-D-mannose 1-phosphate 11% of activity with GDP-mannose Arthrobacter sp. IDPmannose + diphosphate
-
 r
2.7.7.13 ITP + alpha-D-mannose 1-phosphate 11% of activity with GDP-mannose Arthrobacter sp. NRRL B1973 IDPmannose + diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.13 37
-
 assay at Arthrobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.13 48
-
 30 min, 50% loss of activity Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.13 8 9
-
 Arthrobacter sp.