EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.7.12 | expression of wild-type and mutant H166G in strain BL21(DE3) | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.12 | H166G | point mutation leads to shift of the enzyme activity to UDP-hexose synthase activity, formation of uridine 5'-phosphoimidazolate and alpha-D-glucose 1-phosphate from UDP-glucose and imidazole | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.12 | additional information | - |
additional information | kinetics | Escherichia coli | |
2.7.7.12 | additional information | - |
additional information | mutant H166G | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.12 | Escherichia coli | - |
native mutant H166G | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.12 | recombinant from overexpressing strain BL21(DE3) | Escherichia coli |
2.7.7.12 | wild-type and mutant H166G | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.12 | UDP-glucose + alpha-D-galactose 1-phosphate | - |
Escherichia coli | alpha-D-glucose 1-phosphate + UDP-galactose | - |
r | |
2.7.7.12 | UDP-glucose + imidazole | mutant H166G, i.e. UDP-hexose synthase | Escherichia coli | uridine 5'-phosphoimidazole + alpha-D-glucose 1-phosphate | - |
r | |
2.7.7.12 | UDP-glucose + imidazole | equilibrium study and constants | Escherichia coli | uridine 5'-phosphoimidazole + alpha-D-glucose 1-phosphate | - |
r |