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Literature summary extracted from

  • Lee, L.J.; Kimura, A.; Tochikura, T.
    Presence of a single enzyme catalyzing the pyrophosphorolysis of UDP-glucose and UDP-galactose in Bifidobacterium bifidum (1978), Biochim. Biophys. Acta, 527, 301-304.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.9 Bifidobacterium bifidum
-
-
-
2.7.7.10 Bifidobacterium bifidum
-
 glucose-grown
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.9 over 900fold Bifidobacterium bifidum
2.7.7.10 over 900fold Bifidobacterium bifidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.9 15.2
-
 purified enzyme Bifidobacterium bifidum
2.7.7.10 17
-
 purified enzyme Bifidobacterium bifidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.9 additional information UDP-glucose and UDP-galactose pyrophosphorolysis activity in Bifidobacterium bifidum are catalyzed by a single enzyme protein Bifidobacterium bifidum ?
-
 ?
2.7.7.9 UTP + alpha-D-galactose 1-phosphate
-
 Bifidobacterium bifidum diphosphate + UDP-galactose
-
 r
2.7.7.9 UTP + alpha-D-glucose 1-phosphate
-
 Bifidobacterium bifidum diphosphate + UDP-glucose
-
 r
2.7.7.10 diphosphate + UDP-xylose at 10% the rate of UDP-glucose or UDP-galactose Bifidobacterium bifidum ?
-
 r
2.7.7.10 UTP + alpha-D-galactose 1-phosphate reverse reaction at the same rate as UDP-glucose Bifidobacterium bifidum diphosphate + UDP-galactose
-
 r
2.7.7.10 UTP + alpha-D-glucose 1-phosphate reverse reaction at the same rate as UDP-galactose Bifidobacterium bifidum diphosphate + UDP-glucose
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.9 37
-
 assay at Bifidobacterium bifidum
2.7.7.10 37
-
 assay at Bifidobacterium bifidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.9 40
-
 15 min, 10% loss of activity Bifidobacterium bifidum
2.7.7.9 47
-
 t1/2: 10 min Bifidobacterium bifidum
2.7.7.9 60
-
 10 min, inactivation Bifidobacterium bifidum
2.7.7.10 40
-
 15 min, 10% loss of activity Bifidobacterium bifidum
2.7.7.10 47
-
 t1/2: 10 min Bifidobacterium bifidum
2.7.7.10 60
-
 10 min, inactivation Bifidobacterium bifidum