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Literature summary extracted from

  • Sy, J.; Akers, H.
    Purification and properties of guanosine 5,3-polyphosphate synthetase from Bacillus brevis (1976), Biochemistry, 15, 4399-4403.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.5 adenosine 5'-(beta,gamma-Imino)triphosphate
-
 Brevibacillus brevis
2.7.6.5 dGDP
-
 Brevibacillus brevis
2.7.6.5 dGTP
-
 Brevibacillus brevis
2.7.6.5 Mg2+ required as a counter ion for the nucleoside substrate, excess of Mg2+ is inhibitory Brevibacillus brevis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.5 0.14
-
 GDP 30°C, pH 8.1 Brevibacillus brevis
2.7.6.5 0.62
-
 ATP cosubstrate GDP, pH 8.1, 30°C Brevibacillus brevis
2.7.6.5 0.71
-
 ATP cosubstrate GTP, pH 8.1, 30°C Brevibacillus brevis
2.7.6.5 0.77
-
 GTP 30°C, pH 8.1 Brevibacillus brevis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.5 Mg2+ required as a counter-ion for the nucleoside substrate, excess of Mg2+ is inhibitory Brevibacillus brevis
2.7.6.5 Mn2+ can partially replace Mg2+ in activation Brevibacillus brevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.5 55000
-
 sucrose density gradient centrifugation Brevibacillus brevis

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.5 Brevibacillus brevis
-
 ATCC 8185
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.5
-
 Brevibacillus brevis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.5 additional information
-
-
 Brevibacillus brevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.5 2'-methyl-ATP + GTP
-
 Brevibacillus brevis 2'-methyl-AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 8-bromo-ATP + GTP
-
 Brevibacillus brevis 8-bromo-AMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 ATP + GDP preferred substrate Brevibacillus brevis AMP + guanosine 3'-diphosphate 5'-diphosphate i.e ppGpp ?
2.7.6.5 ATP + GTP
-
 Brevibacillus brevis AMP + guanosine 3'-diphosphate 5'-triphosphate i.e. pppGpp ?
2.7.6.5 ATP + guanosine 5'-tetraphosphate
-
 Brevibacillus brevis AMP + guanosine 3'-diphosphate 5'-tetraphosphate
-
 ?
2.7.6.5 ATP + ITP
-
 Brevibacillus brevis AMP + inosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 dATP + GTP
-
 Brevibacillus brevis dAMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.5 additional information no substrate: 5'(beta,gamma-imino)triphosphate, 1,N6-ethyladenosine triphosphate, no diphosphate acceptors: ATP, UTP, CTP, dGTP, dGDP, 2'-O-methyl-GDP, 7-methyl-GDP Brevibacillus brevis ?
-
 ?