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Literature summary extracted from

  • Sonoda, T.; Kita, K.; Ishijima, S.; Ishizuka, T.; Ahmad, I.; Tatibana, M.
    Kinetic and regulatory properties of rat liver phosphoribosylpyrophosphate synthetase complex are partly distinct from those of isolated recombinant component catalytic subunits (1997), J. Biochem., 122, 635-640.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 adenosine 5'-(beta,gamma-imido)-triphosphate inhibition at 1 mM Rattus norvegicus
2.7.6.1 ADP one of the most effective Rattus norvegicus
2.7.6.1 AMP one of the most effective Rattus norvegicus
2.7.6.1 dADP
-
 Rattus norvegicus
2.7.6.1 dATP inhibition at 1 mM Rattus norvegicus
2.7.6.1 GDP one of the most effective Rattus norvegicus
2.7.6.1 additional information native enzyme is less sensitive to nucleotide inhibition than the major component of the enzyme, rPRSI Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.0289
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
2.7.6.1 0.04
-
 D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
2.7.6.1 0.0402
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
2.7.6.1 0.0418
-
 5-phospho-alpha-D-ribose 1-diphosphate pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
2.7.6.1 0.044
-
 ATP pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus
2.7.6.1 0.049
-
 ATP pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
2.7.6.1 0.06
-
 ATP pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
2.7.6.1 0.064
-
 D-ribose 5-phosphate pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
2.7.6.1 0.073
-
 D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
2.7.6.1 0.117
-
 AMP pH 7.4, 37ºC, native enzyme from liver Rattus norvegicus
2.7.6.1 0.123
-
 AMP pH 7.4, 37ºC, isoenzyme rPRSII Rattus norvegicus
2.7.6.1 0.125
-
 AMP pH 7.4, 37ºC, isoenzyme rPRSI Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ requirement Rattus norvegicus
2.7.6.1 Mg2+ activation of liver enzyme is almost maximal at 0.05 mM Rattus norvegicus
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 500000 700000 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 2.45
-
 native enzyme from liver, reverse reaction, pH 8.6 Rattus norvegicus
2.7.6.1 3.59
-
 native enzyme from liver, reverse reaction, pH 7.4 Rattus norvegicus
2.7.6.1 4.24
-
 isoenzyme rPRSII, reverse reaction, pH 8.6 Rattus norvegicus
2.7.6.1 4.5
-
 isoenzyme rPRSI, reverse reaction, pH 8.6 Rattus norvegicus
2.7.6.1 7.48
-
 isoenzyme rPRSI, reverse reaction, pH 7.4 Rattus norvegicus
2.7.6.1 8.04
-
 isoenzyme rPRSII, reverse reaction, pH 7.4 Rattus norvegicus
2.7.6.1 8.3
-
 isoenzyme rPRSII, pH 8.6 Rattus norvegicus
2.7.6.1 16.2
-
 native enzyme from liver, pH 8.6 Rattus norvegicus
2.7.6.1 39.1
-
 isoenzyme rPRSI, pH 8.6 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 4ºC, without stabilizing agents, half-life of inactivation of 21, 8 and 1 hours for native enzyme, isoenzymes rPRSI and rPRSII, respectively Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.1 49
-
 half-life of 250 min, 90 min and 0.5 min for native enzyme, isoenzymes rPRSI and rPRSII, respectively Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.4
-
 rPRSII Rattus norvegicus
2.7.6.1 8.5
-
 native enzyme from liver Rattus norvegicus
2.7.6.1 8.7
-
 rPRSI Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.6.1 7.4
-
 66% of maximal activity, isoenzyme rPRSI Rattus norvegicus
2.7.6.1 7.4
-
 62% of maximal activity, native enzyme from liver Rattus norvegicus
2.7.6.1 8.7
-
 20% of maximal activity, isoenzyme rPRSII Rattus norvegicus