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Literature summary extracted from

  • Becker, M.A.; Kostel, P.J.; Meyer, L.J.
    Human phosphoribosylpyrophosphate synthetase. Comparison of purified normal and mutant enzymes (1975), J. Biol. Chem., 250, 6822-6830.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 2,3-diphosphoglycerate
-
 Homo sapiens
2.7.6.1 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens
2.7.6.1 ADP
-
 Homo sapiens
2.7.6.1 AMP
-
 Homo sapiens
2.7.6.1 GDP
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.03
-
 ATP pH conditions not mentioned, 37ºC, wild type and mutant enzyme Homo sapiens
2.7.6.1 0.03
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.6.1 0.41
-
 D-ribose 5-phosphate pH conditions not mentioned, 37ºC, mutant enzyme Homo sapiens
2.7.6.1 0.57
-
 D-ribose 5-phosphate pH conditions not mentioned, 37ºC, wild type enzyme Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
 enzyme occurs in multiple states of aggregation Homo sapiens
2.7.6.1 32000
-
 x * 32000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 4500fold purification Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 erythrocyte from normal patients and patients with excessive activity of the enzyme Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 3.65
-
 wild type enzyme Homo sapiens
2.7.6.1 8.17
-
 mutant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ? x * 32000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.1 55
-
 inactivation of mutant enzyme proceeds faster than that of normal enzyme Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.1 Homo sapiens isoelectric focusing, mutant enzyme
-
 4.9
2.7.6.1 Homo sapiens isoelectric focusing, normal enzyme
-
 5.1