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Literature summary extracted from

  • Roth, D.G.; Shelton, E.; Deuel, T.F.
    Purification and properties of phosphoribosyl pyrophosphate synthetase from rat liver (1974), J. Biol. Chem., 249, 291-296.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate requirement Rattus norvegicus
2.7.6.1 phosphate optimal activity at 100 mM, slight stabilizing effect Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.6.1 addition of albumin, EDTA or sulfhydryl compounds to the assay prevents loss of activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 D-ribose 5-phosphate substrate inhibition above 1.5 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.078
-
ATP pH 7.6, 37ºC, 100 mM phosphate Rattus norvegicus
2.7.6.1 0.078
-
ATP ATP in form of MgATP2- Rattus norvegicus
2.7.6.1 0.22
-
ATP pH 7.6, 37ºC, excess of Mg2+ Rattus norvegicus
2.7.6.1 0.22
-
ATP ATP in form of MgATP2- Rattus norvegicus
2.7.6.1 0.29
-
5-phospho-D-ribose diphosphate pH 7.6, 37ºC Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Co2+ can partially replace Mg2+ in activation Rattus norvegicus
2.7.6.1 Mg2+ requirement Rattus norvegicus
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Rattus norvegicus
2.7.6.1 Mg2+ the most effective Rattus norvegicus
2.7.6.1 Mn2+ can partially replace Mg2+ Rattus norvegicus
2.7.6.1 additional information absolute requirement for a divalent cation for activity Rattus norvegicus
2.7.6.1 additional information no activity observed with Ca2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
-
Rattus norvegicus
2.7.6.1 40500
-
x * 40500, disc gel electrophoresis in SDS Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Rattus norvegicus
-
rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 acetone powder, acid and streptomycin precipitation, ammonium sulfate fractionation, heat treatment and agarose gel filtration, 3400fold purification, presence of substrates enhances stability during purification Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 liver
-
Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 4ºC, 50% loss of activity after 2 months. -20ºC, more rapid loss of activity. Presence of substrates enhances stability during storage Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 CTP + D-ribose 5-phosphate
-
Rattus norvegicus CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 dATP + D-ribose 5-phosphate slightly more effective than ATP Rattus norvegicus dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 additional information ribose, ribose-1-phosphate, deoxyribose-5-phosphate, glucose-6-phosphate are not effective as substrates Rattus norvegicus ?
-
?
2.7.6.1 UTP + D-ribose 5-phosphate
-
Rattus norvegicus UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ? x * 40500, disc gel electrophoresis in SDS Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8 8.8
-
Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.6.1 7
-
the enzyme retains over 70% of the activity Rattus norvegicus