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Literature summary extracted from

  • Ishijima, S.; Kita, K.; Ahmad, I.; Ishizuka, T.; Taira, M.; Tatibana, M.
    Expression of rat phosphoribosylpyrophosphate synthetase subunits I and II in Escherichia coli. Isolation and characterization of the recombinant isoforms (1991), J. Biol. Chem., 266, 15693-15697.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 additional information at 50 and 100 mM, KCl, KHCO3 and CH3COOK have no activating effects Rattus norvegicus
2.7.6.1 phosphate requirement Rattus norvegicus
2.7.6.1 phosphate no activity at phosphate concentrations lower than 0.4 mM Rattus norvegicus
2.7.6.1 sulfate can partially replace phosphate in activation Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.1 expression in Escherichia coli Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP one of the most effective Rattus norvegicus
2.7.6.1 AMP one of the most effective Rattus norvegicus
2.7.6.1 CTP no inhibition Rattus norvegicus
2.7.6.1 GDP one of the most effective Rattus norvegicus
2.7.6.1 GMP no inhibition Rattus norvegicus
2.7.6.1 GTP no inhibition Rattus norvegicus
2.7.6.1 UDP no inhibition Rattus norvegicus
2.7.6.1 UMP no inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.04
-
 D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme PRSI Rattus norvegicus
2.7.6.1 0.044
-
 ATP pH 7.4, 37ºC, isoenzyme PRSI Rattus norvegicus
2.7.6.1 0.06
-
 ATP pH 7.4, 37ºC, isoenzyme PRSII Rattus norvegicus
2.7.6.1 0.073
-
 D-ribose 5-phosphate pH 7.4, 37ºC, isoenzyme PRSII Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 34000
-
 x * 34000, SDS-PAGE, cloned and purified isoenzymes PRSI and PRSII Rattus norvegicus
2.7.6.1 550000
-
 gel filtration, cloned and purified rPRSII Rattus norvegicus
2.7.6.1 700000 1200000 gel filtration, cloned and purified rPRSI Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Rattus norvegicus
-
 rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 polyethylene glycol, acid precipitation and chromatography on DEAE-5PW HPLC, purification of the two isoforms PRSI and PRSII Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 25.7
-
 isoenzyme PRSI Rattus norvegicus
2.7.6.1 34.5
-
 isoenzyme PRSII Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 -80ºC, potassium phosphate 50 mM, pH 7.4 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ? x * 34000, SDS-PAGE, cloned and purified isoenzymes PRSI and PRSII Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.6.1 49
-
 half-life of 0.5 min and 90 min for isoenzymes rPRSII and rPRSI, respectively Rattus norvegicus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.1 Rattus norvegicus isoenzyme rPRSII 4.4 4.3
2.7.6.1 Rattus norvegicus isoenzyme rPRSI
-
 4.4