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Literature summary extracted from

  • Switzer, R.L.; Gibson, K.J.
    Phosphoribosylpyrophosphate synthetase (ribose-5-phosphate pyrophosphokinase) from Salmonella typhimurium (1978), Methods Enzymol., 51, 3-11.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 phosphate specific and kinetically complex requirement, 25-50 mM required for optimal 5-phospho-alpha-D-ribose 1-diphosphate synthesis, 5 mM optimal for reverse reaction Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
2.7.6.1 repeated freezing and thawing slowly denatures the enzyme. Irreversible denaturation by reduction of the phosphate concentration to less than 5 mM, requires 25 mM phosphate or higher for complete stability. Mg2+ ions and ATP stabilize the enzyme but will not replace phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ADP the most effective Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 alpha,beta-methyleneATP competitive inhibition to ATP Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Ca2+ IC50 of 0.05 mM; inhibition even in presence of excess Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Cd2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Cu2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 D-ribose 5-phosphate substrate inhibition in presence of ADP, not in its absence Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 DL-1,4-Anhydroribitol 5-phosphate competitive inhibition to D-ribose-5-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information Ba2+, Fe2+, Mn2+ and Zn2+ do not inhibit at concentrations below 1 mM; purines are more effective than pyrimidines, nucleoside tri and diphosphates are more effective than monophosphates, ribonucleotides are more effective than deoxynucleotides Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Ni2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 nucleotides
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.046
-
 ATP pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.046
-
 ATP ATP in form of MgATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.16
-
 D-ribose 5-phosphate pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.29
-
 5-phospho-D-ribose diphosphate pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.32
-
 AMP pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Mn2+ equally effective as Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
 enzyme occurs in multiple states of aggregation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 31000
-
 5 * 31000, physical and electron microscopic studies Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 160000
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2
-
2.7.6.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
 Su 422 strain
-
2.7.6.1 Salmonella enterica subsp. enterica serovar Typhimurium Su 422
-
 Su 422 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, affinity chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 130
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 -20°C, 50 mM potassium phosphate buffer, pH 7.5 after rapid freezing in CO2-acetone, quite stable Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate maximal velocity of the reverse reaction is 9-10% of the forward reaction Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Salmonella enterica subsp. enterica serovar Typhimurium Su 422 AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate maximal velocity of the reverse reaction is 9-10% of the forward reaction Salmonella enterica subsp. enterica serovar Typhimurium Su 422 AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 CTP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 CTP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium Su 422 CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 GTP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 GTP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium Su 422 GMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ITP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium IMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 UTP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 UTP + D-ribose 5-phosphate less than 3% of the activity with ATP Salmonella enterica subsp. enterica serovar Typhimurium Su 422 UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ?
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 pentamer 5 * 31000, physical and electron microscopic studies Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8 8.6
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.6.1 0.03
-
 alpha,beta-methyleneATP pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 10
-
 DL-1,4-Anhydroribitol 5-phosphate pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.6.1 0.05
-
 IC50 of 0.05 mM Salmonella enterica subsp. enterica serovar Typhimurium Ca2+