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Literature summary extracted from

  • Switzer, R.L.
    Phosphoribosylpyrophosphate synthetase and related pyrophosphokinases (1974), The Enzymes,3rd Ed. (Boyer,P. D. ,ed. ), 10, 607-629.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 EDTA prevents inhibition by traces of cations Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate activates at low concentrations Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate specific and absolute requirement Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate specific and absolute requirement Mus musculus
2.7.6.1 phosphate specific and absolute requirement Homo sapiens
2.7.6.1 phosphate specific and absolute requirement Rattus norvegicus
2.7.6.1 phosphate appears to have an effect on the stability of the enzyme as well as a specific catalytic effect Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate appears to have an effect on the stability of the enzyme as well as a specific catalytic effect Homo sapiens

General Stability

EC Number General Stability Organism
2.7.6.1 purified enzyme, quite stable Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 sulfhydryl compounds inactivate highly purified PRPP synthetase from erythrocytes, essential for stability and assay of partially purified enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 2,3-diphosphoglycerate competitive to D-ribose 5-phosphate; inhibition of the erythrocyte enzyme Homo sapiens
2.7.6.1 ADP
-
 Columba sp.
2.7.6.1 ADP
-
 Escherichia coli
2.7.6.1 ADP
-
 Gallus gallus
2.7.6.1 ADP competitive inhibition to ATP Homo sapiens
2.7.6.1 ADP
-
 Mus musculus
2.7.6.1 ADP
-
 Ophiodon elongatus
2.7.6.1 ADP competitive inhibition to ATP Rattus norvegicus
2.7.6.1 ADP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 alpha,beta-methyleneATP competitive inhibition to ATP Homo sapiens
2.7.6.1 alpha,beta-methyleneATP competitive inhibition to ATP Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Ca2+ inhibition even in presence of excess Mg2+ Escherichia coli
2.7.6.1 Ca2+
-
 Homo sapiens
2.7.6.1 Ca2+ inhibition even in presence of excess Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 D-ribose 5-phosphate
-
 Escherichia coli
2.7.6.1 D-ribose 5-phosphate
-
 Rattus norvegicus
2.7.6.1 D-ribose 5-phosphate inhibition in presence of Ca2+, without ADP; substrate inhibition in presence of ADP, not in its absence Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 L-histidine weak inhibition or no inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 L-tryptophan weak inhibition or no inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information
-
 Homo sapiens
2.7.6.1 additional information
-
 Rattus norvegicus
2.7.6.1 additional information purines are more effective than pyrimidines, nucleoside tri and diphosphates are more effective than monophosphates, ribonucleotides are more effective than deoxynucleotides Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 nucleotides
-
 Rattus norvegicus
2.7.6.1 nucleotides nucleoside tri- and diphosphates are more effective than nucleoside monophosphates, ribonucleotides more effective than deoxynucleotides; purines more effective than pyrimidines Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate inhibition at high concentrations, more pronounced in the reverse reaction Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 additional information
-
 additional information increasing phosphate concentrations, increases Km values for ATP and D-ribose-5-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.014
-
 ATP pH and temperature conditions not mentioned Homo sapiens
2.7.6.1 0.014
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.6.1 0.033
-
 ribose 5-phosphate pH and temperature conditions not mentioned Homo sapiens
2.7.6.1 0.046
-
 ATP pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.046
-
 ATP ATP in form of MgATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.16
-
 D-ribose 5-phosphate pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.2
-
 Mg2+ pH and temperature conditions not mentioned Homo sapiens
2.7.6.1 0.6
-
 Mg2+ pH and temperature conditions not mentioned Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.1 mitochondrion 80% of the liver enzyme is found in the mitochondria Columba sp. 5739
-
2.7.6.1 nucleus 15% of the liver enzyme is found in the nucleus Columba sp. 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Mg2+
-
 Gallus gallus
2.7.6.1 Mg2+
-
 Mus musculus
2.7.6.1 Mg2+
-
 Escherichia coli
2.7.6.1 Mg2+
-
 Ophiodon elongatus
2.7.6.1 Mg2+
-
 Columba sp.
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Homo sapiens
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Rattus norvegicus
2.7.6.1 Mg2+ the most effective Homo sapiens
2.7.6.1 Mg2+ the most effective Rattus norvegicus
2.7.6.1 Mn2+ equally effective as Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information absolute requirement for a divalent cation for activity Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information absolute requirement for a divalent cation for activity Homo sapiens
2.7.6.1 additional information absolute requirement for a divalent cation for activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 additional information
-
-
 Rattus norvegicus
2.7.6.1 additional information
-
 enzyme occurs in multiple states of aggregation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information
-
 enzyme occurs in multiple states of aggregation Homo sapiens
2.7.6.1 additional information
-
 enzyme occurs in multiple states of aggregation Columba sp.
2.7.6.1 additional information
-
 the distribution among the states of aggregation is altered by a variety of conditions Columba sp.
2.7.6.1 34500
-
 x * 34500, SDS-PAGE Homo sapiens
2.7.6.1 43000
-
 x * 43000, sucrose density gradient sedimentation in the presence of urea 2 M combined with values of gel filtration Homo sapiens
2.7.6.1 53000
-
 x * 53000, sucrose density gradient sedimentation in the presence of NaCl, 1 M, combined with values of gel filtration Homo sapiens
2.7.6.1 60000
-
 x * 60000, sucrose density gradient sedimentation in phosphate buffer combined with values of gel filtration Homo sapiens
2.7.6.1 250000 600000 sucrose density gradient sedimentation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 700000 1200000
-
 Rattus norvegicus
2.7.6.1 700000 1200000 sucrose density gradient sedimentation and gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate Escherichia coli
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate Homo sapiens
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate Rattus norvegicus
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate Columba sp.
-
 AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Columba sp.
-
-
-
2.7.6.1 Escherichia coli
-
-
-
2.7.6.1 Gallus gallus
-
 chicken
-
2.7.6.1 Homo sapiens
-
-
-
2.7.6.1 Mus musculus
-
 mouse
-
2.7.6.1 Ophiodon elongatus
-
 lingcod
-
2.7.6.1 Rattus norvegicus
-
 rat
-
2.7.6.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 ammonium sulfate and isoelectric precipitations, purified to near homogeneity Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 to near homogeneity. Use of Mg2+, ATP and sulfhydryl compounds to stabilize the enzyme permit its isolation from erythrocytes Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Salmonella enterica subsp. enterica serovar Typhimurium
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Gallus gallus
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Mus musculus
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Escherichia coli
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Homo sapiens
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Rattus norvegicus
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Ophiodon elongatus
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Columba sp.
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction with ribose-5-phosphate binding first and 5-phosphoribose diphosphate being released last Salmonella enterica subsp. enterica serovar Typhimurium
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction with ribose-5-phosphate binding first and 5-phosphoribose diphosphate being released last Homo sapiens
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Salmonella enterica subsp. enterica serovar Typhimurium
a
2.7.6.1 ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi reaction Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 Ehrlich ascites carcinoma cell tumor cell line Mus musculus
-
2.7.6.1 erythrocyte
-
 Homo sapiens
-
2.7.6.1 liver
-
 Gallus gallus
-
2.7.6.1 liver
-
 Mus musculus
-
2.7.6.1 liver
-
 Rattus norvegicus
-
2.7.6.1 liver
-
 Columba sp.
-
2.7.6.1 muscle
-
 Ophiodon elongatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + 6-deoxyhomoribose 6-phosphonate
-
 Mus musculus AMP + 6-deoxyhomoribose 6-phosphonate 1-diphosphate
-
 r
2.7.6.1 ATP + 6-deoxyhomoribose 6-phosphonate
-
 Homo sapiens AMP + 6-deoxyhomoribose 6-phosphonate 1-diphosphate
-
 r
2.7.6.1 ATP + 6-deoxyhomoribose 6-phosphonate
-
 Rattus norvegicus AMP + 6-deoxyhomoribose 6-phosphonate 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Escherichia coli AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Columba sp. AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Gallus gallus AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Escherichia coli AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Ophiodon elongatus AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Columba sp. AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Mus musculus AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for ATP Columba sp. AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for D-ribose 5-phosphate Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for D-ribose 5-phosphate Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for D-ribose 5-phosphate Homo sapiens AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate highly specific for D-ribose 5-phosphate Rattus norvegicus AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphate maximal velocity of the reverse reaction is 9-10% of the forward reaction Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as PRPP r
2.7.6.1 ATP + D-ribose 5-phosphate maximal velocity of the reverse reaction is 9-10% of the forward reaction Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate ribose product also known as 5-phosphoryl-D-ribofuranose alpha-1-diphosphate r
2.7.6.1 ATP + D-ribose 5-phosphothionate
-
 Mus musculus AMP + 5-phosphotionate-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphothionate
-
 Homo sapiens AMP + 5-phosphotionate-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 ATP + D-ribose 5-phosphothionate
-
 Rattus norvegicus AMP + 5-phosphotionate-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 CTP + D-ribose 5-phosphate does not serve as substrate Salmonella enterica subsp. enterica serovar Typhimurium CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 CTP + D-ribose 5-phosphate does not serve as substrate Mus musculus CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 CTP + D-ribose 5-phosphate does not serve as substrate Homo sapiens CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 CTP + D-ribose 5-phosphate does not serve as substrate Columba sp. CMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 dATP + D-ribose 5-phosphate
-
 Rattus norvegicus dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 dATP + D-ribose 5-phosphate in erythrocyte enzyme and tumor cell enzyme Mus musculus dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 dATP + D-ribose 5-phosphate in erythrocyte enzyme and tumor cell enzyme Homo sapiens dAMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
2.7.6.1 additional information
-
 Rattus norvegicus ?
-
 ?
2.7.6.1 additional information 2-deoxyribose-5-phosphate is not substrate Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.6.1 additional information D-ribitol-5-phosphate, alpha-methyl-D-ribofuranoside-5-phosphate, beta-methyl-D-ribofuranoside-5-phosphate are no substrates Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.6.1 additional information other nucleosides di and triphosphates than ATP do not serve as substrates Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.6.1 additional information other nucleosides di and triphosphates than ATP do not serve as substrates Mus musculus ?
-
 ?
2.7.6.1 additional information other nucleosides di and triphosphates than ATP do not serve as substrates Homo sapiens ?
-
 ?
2.7.6.1 additional information other nucleosides di and triphosphates than ATP do not serve as substrates Columba sp. ?
-
 ?
2.7.6.1 UTP + D-ribose 5-phosphate does not serve as substrate Salmonella enterica subsp. enterica serovar Typhimurium UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 UTP + D-ribose 5-phosphate does not serve as substrate Mus musculus UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 UTP + D-ribose 5-phosphate does not serve as substrate Homo sapiens UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?
2.7.6.1 UTP + D-ribose 5-phosphate does not serve as substrate Columba sp. UMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.1 ?
-
 Rattus norvegicus
2.7.6.1 ? x * 32000-35000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 ? x * 34500, SDS-PAGE Homo sapiens
2.7.6.1 ? x * 43000, sucrose density gradient sedimentation in the presence of urea 2 M combined with values of gel filtration Homo sapiens
2.7.6.1 ? x * 53000, sucrose density gradient sedimentation in the presence of NaCl, 1 M, combined with values of gel filtration Homo sapiens
2.7.6.1 ? x * 60000, sucrose density gradient sedimentation in phosphate buffer combined with values of gel filtration Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.2 7.4 erythrocyte enzyme Homo sapiens
2.7.6.1 8 8.6
-
 Salmonella enterica subsp. enterica serovar Typhimurium