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Literature summary extracted from

  • Ashihara, H.
    Characterization of phosphoribosylpyrophosphate synthetase from spinach leaves (1977), Z. Pflanzenphysiol., 83, 379-392.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 phosphate IC50 of 28 mM Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.1 cytosol more than 95% of the total activity Spinacia oleracea 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Co2+ can partially replace Mg2+ in activation Spinacia oleracea
2.7.6.1 Mg2+ requirement Spinacia oleracea
2.7.6.1 Mn2+ can partially replace Mg2+ Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Spinacia oleracea
-
 spinach
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 ammonium sulfate fractionation, chromatography on DEAE-cellulose and ultrafiltration Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.6.1 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 additional information
-
 10fold greater in cytosol than in the particulate fraction Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
 Spinacia oleracea AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 7.6
-
-
 Spinacia oleracea

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.6.1 28
-
 IC50 of 28 mM Spinacia oleracea phosphate