EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | 1-Aminoethylphosphonate | 0.1 M, 13% of the activity with phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | arsenate | 0.1 M, 43% of the activity with phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | phosphate | specific and absolute requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | phosphate | appears to have an effect on the stability of the enzyme as well as a specific catalytic effect | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | phosphate | the enzyme has 2 sites for phosphate, one of high affinity and one of lower affinity | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | General Stability | Organism |
---|---|---|
2.7.6.1 | completely inactivated when phosphate is removed by dialysis | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | ATP | free form, slight inhibition at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | GSH | marked inhibition of enzyme in crude extract at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | additional information | GSH, 2-mercaptoethanol, 2,3-dimercaptopropanol, dithiothreitol, at 1 mM, have no effect or slightly inhibit the highly purified enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.6.1 | 0.05 | 0.13 | D-ribose 5-phosphate | pH 7.5, 37ºC | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.06 | - |
ATP | pH 7.5, 37ºC, excess of Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.06 | - |
ATP | ATP in form of MnATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.1 | - |
ATP | pH 7.5, 37ºC, excess of Mg2+, bimodal kinetic behavior | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.1 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.15 | 0.4 | AMP | pH 7.5, 37ºC | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.35 | - |
ATP | pH 7.5, 37ºC, no excess of Mn2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.35 | - |
ATP | ATP in form of MnATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.58 | - |
ATP | pH 7.5, 37ºC, excess of Mg2+, bimodal kinetic behavior | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 0.58 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 2.3 | - |
phosphate | pH 7.5, 37ºC, high affinity site | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 2.9 | - |
ATP | pH 7.5, 37ºC, no excess of Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 2.9 | - |
ATP | ATP in form of MgATP2- | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | 40 | - |
phosphate | pH 7.5, 37ºC, low affinity site | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.6.1 | Co2+ | no activity observed | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | Mg2+ | requirement | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | Mg2+ | required to form a complex with ATP and as a free cation | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | Mn2+ | equally effective as Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | additional information | absolute requirement for a divalent cation for activity | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | additional information | no activity observed with Ca2+ | Salmonella enterica subsp. enterica serovar Typhimurium | |
2.7.6.1 | Zn2+ | 25% of the activity with Mg2+ | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 540000 | - |
sucrose density gradient sedimentation | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.6.1 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
LT-2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.6.1 | extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, 450fold purification | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | additional information | - |
26.8 U/mg protein, purified enzyme, 400fold to 450fold the activity of crude extract | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.6.1 | 0°C or -10°C, 50 mM potassium phosphate buffer, pH 7.4, stable for weeks | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.6.1 | ATP + D-ribose 5-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
2.7.6.1 | ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Salmonella enterica subsp. enterica serovar Typhimurium | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 8.1 | 8.6 | for either the Mg2+ or the Mn2+ supported reaction | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.6.1 | 6 | 9.5 | very low activity below and above | Salmonella enterica subsp. enterica serovar Typhimurium |