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Literature summary extracted from

  • Switzer, R.L.
    Regulation and mechanism of phosphoribosylpyrophosphate synthetase. I. Purification and properties of the enzyme from Salmonella typhimurium (1969), J. Biol. Chem., 244, 2854-2863.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.6.1 1-Aminoethylphosphonate 0.1 M, 13% of the activity with phosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 arsenate 0.1 M, 43% of the activity with phosphate Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate specific and absolute requirement Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate appears to have an effect on the stability of the enzyme as well as a specific catalytic effect Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 phosphate the enzyme has 2 sites for phosphate, one of high affinity and one of lower affinity Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

EC Number General Stability Organism
2.7.6.1 completely inactivated when phosphate is removed by dialysis Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.1 ATP free form, slight inhibition at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 GSH marked inhibition of enzyme in crude extract at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information GSH, 2-mercaptoethanol, 2,3-dimercaptopropanol, dithiothreitol, at 1 mM, have no effect or slightly inhibit the highly purified enzyme Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.6.1 0.05 0.13 D-ribose 5-phosphate pH 7.5, 37ºC Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.06
-
ATP pH 7.5, 37ºC, excess of Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.06
-
ATP ATP in form of MnATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.1
-
ATP pH 7.5, 37ºC, excess of Mg2+, bimodal kinetic behavior Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.1
-
ATP ATP in form of MgATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.15 0.4 AMP pH 7.5, 37ºC Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.35
-
ATP pH 7.5, 37ºC, no excess of Mn2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.35
-
ATP ATP in form of MnATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.58
-
ATP pH 7.5, 37ºC, excess of Mg2+, bimodal kinetic behavior Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 0.58
-
ATP ATP in form of MgATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 2.3
-
phosphate pH 7.5, 37ºC, high affinity site Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 2.9
-
ATP pH 7.5, 37ºC, no excess of Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 2.9
-
ATP ATP in form of MgATP2- Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 40
-
phosphate pH 7.5, 37ºC, low affinity site Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.1 Co2+ no activity observed Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Mg2+ requirement Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Mg2+ required to form a complex with ATP and as a free cation Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Mn2+ equally effective as Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information absolute requirement for a divalent cation for activity Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 additional information no activity observed with Ca2+ Salmonella enterica subsp. enterica serovar Typhimurium
2.7.6.1 Zn2+ 25% of the activity with Mg2+ Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.1 540000
-
sucrose density gradient sedimentation Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate Salmonella enterica subsp. enterica serovar Typhimurium the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
LT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.1 extraction, streptomycin-heat treatment, ammonium sulfate and acid precipitation, 450fold purification Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.6.1 additional information
-
26.8 U/mg protein, purified enzyme, 400fold to 450fold the activity of crude extract Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.6.1 0°C or -10°C, 50 mM potassium phosphate buffer, pH 7.4, stable for weeks Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.1 ATP + D-ribose 5-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
2.7.6.1 ATP + D-ribose 5-phosphate the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan Salmonella enterica subsp. enterica serovar Typhimurium AMP + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.6.1 37
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.1 8.1 8.6 for either the Mg2+ or the Mn2+ supported reaction Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.6.1 6 9.5 very low activity below and above Salmonella enterica subsp. enterica serovar Typhimurium