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Literature summary extracted from

  • Navé, J.F.; Eschbach, A.; Halazy, S.
    9-(Phosphonoalkyl)guanine derivatives as substrates or inhibitors of guanylate kinase (1992), Arch. Biochem. Biophys., 295, 253-257.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 9-(6,6-difluoro-6-phosphonohexyl)guanine competitive with respect to GMP, non-competitive with respect to ATP Sus scrofa
2.7.4.8 9-(6-phosphonohexyl)guanine competitive with respect to GMP, non-competitive with respect to ATP Sus scrofa
2.7.4.8 additional information no inhibition by 9-(5-phosphonopentyl)guanine (i.e. isosteric analogue of acyclovir 5'-monophosphate) Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.032
-
 GMP pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.083
-
 dGMP pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.25
-
 9-(5-phosphonopentyl)guanine pH 7.5, 30ºC Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Mg2+
-
 Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.8 ATP + GMP Sus scrofa key enzyme of biosynthetic pathway of GTP or dGTP ADP + GDP
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Sus scrofa
-
 pig
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 brain
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5'-monophosphate no substrate: 9-(5,5-difluoro-5-phosphonopentyl)guanine 5'-monophosphate Sus scrofa ADP + 9-(1,3-dihydroxy-2-propoxymethyl)guanine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 9-(2-hydroxyethoxymethyl)guanine 5'-monophosphate i.e. acyclovir 5'-monophosphate Sus scrofa ADP + 9-(2-hydroxyethoxymethyl)guanine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 9-(5-phosphonopentyl)guanine 9-(5,5'-difluoro-5-phosphonopenthyl)guanine is not a substrate Sus scrofa ?
-
 ?
2.7.4.8 ATP + dGMP
-
 Sus scrofa ADP + dGDP
-
 r
2.7.4.8 ATP + GMP
-
 Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP key enzyme of biosynthetic pathway of GTP or dGTP Sus scrofa ADP + GDP
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7.5
-
 assay at Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.8 0.11
-
 9-(6-phosphonohexyl)guanine pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.14
-
 9-(6,6-difluoro-6-phosphonohexyl)guanine pH 7.5, 30ºC, higher affinity than 9-(6-phosphonohexyl)guanine for guanylate kinases al pH values below 7.5 Sus scrofa