Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Agarwal, K.C.; Miech, R.P.; Parks, R.E.
    Guanylate kinases from human erythrocytes, hog brain, and rat liver (1978), Methods Enzymol., 51, 483-490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 5,5'-dithiobis(2-nitrobenzoate)
-
 Homo sapiens
2.7.4.8 5,5'-dithiobis(2-nitrobenzoate)
-
 Rattus norvegicus
2.7.4.8 additional information no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate Homo sapiens
2.7.4.8 additional information no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate Rattus norvegicus
2.7.4.8 additional information no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol; no inhibition by guanosine, AMP, CMP, UMP, XMP, 6-thioinosine 5'-phosphate Sus scrofa
2.7.4.8 N-ethylmaleimide
-
 Homo sapiens
2.7.4.8 N-ethylmaleimide
-
 Rattus norvegicus
2.7.4.8 p-chloromercuribenzoic acid 1,4-dithiothreitol reverses Homo sapiens
2.7.4.8 p-chloromercuribenzoic acid 1,4-dithiothreitol reverses Rattus norvegicus
2.7.4.8 p-chloromercuribenzoic acid 1,4-dithiothreitol reverses Sus scrofa
2.7.4.8 p-hydroxymercuribenzoate
-
 Homo sapiens
2.7.4.8 p-hydroxymercuribenzoate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.006
-
 GMP pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.01
-
 GMP pH 7.5, 30ºC Rattus norvegicus
2.7.4.8 0.01
-
 dGMP pH 7.5, 30ºC Rattus norvegicus
2.7.4.8 0.01
-
 dGMP pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.013
-
 8-azaguanosine 5'-monophosphate pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.018
-
 GMP pH 7.5, 30ºC Homo sapiens
2.7.4.8 0.028
-
 dGMP pH 7.5, 30ºC Rattus norvegicus
2.7.4.8 0.07
-
 8-azaguanosine 5'-monophosphate pH 7.5, 30ºC Rattus norvegicus
2.7.4.8 0.074
-
 dGMP pH 7.5, 30ºC Homo sapiens
2.7.4.8 0.091
-
 8-azaguanosine 5'-monophosphate pH 7.5, 30ºC Homo sapiens
2.7.4.8 0.12
-
 ATP pH 7.5, 30ºC Sus scrofa
2.7.4.8 0.18
-
 ATP pH 7.5, 30ºC Rattus norvegicus
2.7.4.8 0.19
-
 ATP pH 7.5, 30ºC Homo sapiens
2.7.4.8 2.1
-
 6-thioguanosine 5'-monophosphate pH 7.5, 30ºC Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Ca2+ no activator Homo sapiens
2.7.4.8 Ca2+ no activator Rattus norvegicus
2.7.4.8 Ca2+ no activator Sus scrofa
2.7.4.8 K+ activation Homo sapiens
2.7.4.8 K+ activation Rattus norvegicus
2.7.4.8 K+ activation Sus scrofa
2.7.4.8 Li+ partial activity Sus scrofa
2.7.4.8 Mg2+
-
 Homo sapiens
2.7.4.8 Mg2+
-
 Rattus norvegicus
2.7.4.8 Mg2+
-
 Sus scrofa
2.7.4.8 Mn2+ requirement Homo sapiens
2.7.4.8 Mn2+ requirement Rattus norvegicus
2.7.4.8 Mn2+ requirement Sus scrofa
2.7.4.8 additional information
-
 Homo sapiens
2.7.4.8 additional information
-
 Rattus norvegicus
2.7.4.8 additional information no activation by Sr2+ or Cs2+ Sus scrofa
2.7.4.8 Na+ activation Sus scrofa
2.7.4.8 NH4+ activation Homo sapiens
2.7.4.8 NH4+ activation Rattus norvegicus
2.7.4.8 NH4+ activation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Homo sapiens
-
-
-
2.7.4.8 Rattus norvegicus
-
-
-
2.7.4.8 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 method that includes DEAE-cellulose and Sephadex-75 chromatography Rattus norvegicus
2.7.4.8 method that includes DEAE-cellulose and Sephadex-75 chromatography Sus scrofa
2.7.4.8 method that includes DEAE-cellulose, Sephadex-75 chromatography and isoelectric focusing Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 brain
-
 Rattus norvegicus
-
2.7.4.8 brain
-
 Sus scrofa
-
2.7.4.8 erythrocyte
-
 Homo sapiens
-
2.7.4.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.8 28
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 0°C, below, in 70% saturated ammonium sulfate, several years Homo sapiens
2.7.4.8 0°C, below, in 70% saturated ammonium sulfate, several years Rattus norvegicus
2.7.4.8 0°C, below, in 70% saturated ammonium sulfate, several years Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + 6-thioguanosine 5'-monophosphate
-
 Homo sapiens ADP + 8-thioguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 6-thioguanosine 5'-monophosphate
-
 Rattus norvegicus ADP + 8-thioguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 6-thioguanosine 5'-monophosphate
-
 Sus scrofa ADP + 8-thioguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 8-azaguanosine 5'-monophosphate
-
 Homo sapiens ADP + 8-azaguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 8-azaguanosine 5'-monophosphate
-
 Rattus norvegicus ADP + 8-azaguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 8-azaguanosine 5'-monophosphate
-
 Sus scrofa ADP + 8-azaguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + dGMP
-
 Homo sapiens ADP + dGDP
-
 r
2.7.4.8 ATP + dGMP
-
 Rattus norvegicus ADP + dGDP
-
 r
2.7.4.8 ATP + dGMP
-
 Sus scrofa ADP + dGDP
-
 r
2.7.4.8 ATP + GMP specificity Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP specificity Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP specificity Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP nucleoside monophosphate binding site is highly specific for guanine moiety Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP nucleoside monophosphate binding site is highly specific for guanine moiety Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP nucleoside monophosphate binding site is highly specific for guanine moiety Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP 6-thio-IMP is no acceptor substrate Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP 6-thio-IMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP 6-thio-IMP is no acceptor substrate Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP XMP is no acceptor substrate Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP XMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP XMP is no acceptor substrate Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Homo sapiens ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Sus scrofa ADP + GDP
-
 ?
2.7.4.8 ATP + IMP very poor substrate Homo sapiens ADP + IDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Homo sapiens dADP + dGDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Rattus norvegicus dADP + dGDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Sus scrofa dADP + dGDP
-
 ?
2.7.4.8 dATP + GMP
-
 Homo sapiens dADP + GDP
-
 ?
2.7.4.8 dATP + GMP
-
 Rattus norvegicus dADP + GDP
-
 ?
2.7.4.8 dATP + GMP
-
 Sus scrofa dADP + GDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 30
-
 assay at Homo sapiens
2.7.4.8 30
-
 assay at Rattus norvegicus
2.7.4.8 30
-
 assay at Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.8 30
-
 at least 15 min stable from pH 5.5-8.5 Sus scrofa
2.7.4.8 50
-
 10 min, inactivation Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.8 5.5 8.5 at least 15 min stable at 30°C Sus scrofa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.4.8 Rattus norvegicus 3 isoelectric variants, predominant form: pI 4.9, agarose gel electrophoresis and isoelectric focusing 4.8 4.7
2.7.4.8 Homo sapiens 4 isoelectric variants, agarose gel electrophoresis and isoelectric focusing
-
 4.9