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Literature summary extracted from

  • Buccino, R.J.; Roth, J.S.
    Partial purification and properties of ATP:GMP phosphransferase from rat liver (1969), Arch. Biochem. Biophys., 132, 49-61.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.8 additional information no activation by 1,4-dithiothreitol or 2-mercaptoethanol Rattus norvegicus

General Stability

EC Number General Stability Organism
2.7.4.8 2-mercaptoethanol does not stabilize Rattus norvegicus
2.7.4.8 freeze-thawing rapidly inactivates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 5,5'-dithiobis(2-nitrobenzoate)
-
 Rattus norvegicus
2.7.4.8 6-Thioguanosine 5'-phosphate dGMP-phosphorylation; not thiodeoxyguanosine derivative Rattus norvegicus
2.7.4.8 8-azaguanosine 5'-monophosphate (d)GMP-phosphorylation Rattus norvegicus
2.7.4.8 8-bromoguanosine 5'-monophosphate (d)GMP-phosphorylation Rattus norvegicus
2.7.4.8 Cs+ strong Rattus norvegicus
2.7.4.8 indol-3-acetic acid GMP + ATP protect Rattus norvegicus
2.7.4.8 Li+ strong Rattus norvegicus
2.7.4.8 additional information GMP-phosphorylation is less sensitive to metal ions than dGMP-phosphorylation; minimal inactivation by iodoacetate and iodoacetamide not affected by the presence or absence of KCl; no inhibition by 2-mercaptoethanol, 1,4-dithiothreitol Rattus norvegicus
2.7.4.8 N-ethylmaleimide
-
 Rattus norvegicus
2.7.4.8 p-hydroxymercuribenzoate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Ca2+ activation Rattus norvegicus
2.7.4.8 K+ activation Rattus norvegicus
2.7.4.8 K+ dGMP, not GMP as substrate Rattus norvegicus
2.7.4.8 Mg2+ requirement Rattus norvegicus
2.7.4.8 Mg2+ active substrate: MgATP2- Rattus norvegicus
2.7.4.8 additional information
-
 Rattus norvegicus
2.7.4.8 Na+ no activator Rattus norvegicus
2.7.4.8 NH4+ activation Rattus norvegicus
2.7.4.8 NH4+ less effective than K+ Rattus norvegicus
2.7.4.8 Rb+ activation, less effective than K+ Rattus norvegicus
2.7.4.8 Zn2+ activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.8 20500
-
 gel filtration Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Rattus norvegicus
-
 female Fischer or male Buffalo rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 partial by a method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-200 chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 hepatoma cell Morris 7793 or Dunning Rattus norvegicus
-
2.7.4.8 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.8 3.2
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 -15°C, more than 6 months Rattus norvegicus
2.7.4.8 0-4°C, 6-10 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + 6-thioguanosine 5'-monophosphate not thiodeoxyguanosine derivative Rattus norvegicus ADP + 8-thioguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 8-azaguanosine 5'-monophosphate
-
 Rattus norvegicus ADP + 8-azaguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + 8-bromoguanosine 5'-monophosphate poor substrate Rattus norvegicus ADP + 8-bromoguanosine 5'-diphosphate
-
 ?
2.7.4.8 ATP + dGMP
-
 Rattus norvegicus ADP + dGDP
-
 r
2.7.4.8 ATP + GMP specificity Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP deoxyguanosine, guanosine are no acceptor substrates Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP TMP is not an acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dCMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP IMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP XMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dAMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 dATP + dGMP
-
 Rattus norvegicus dADP + dGDP
-
 ?
2.7.4.8 dATP + GMP
-
 Rattus norvegicus dADP + GDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.8 37
-
 assay at Rattus norvegicus