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Literature summary extracted from

  • Shimono, H.; Sugino, Y.
    Metabolism of deoxyribonucleotides. Purification and properties of deoxyguanosine monophosphokinase of calf thymus (1971), Eur. J. Biochem., 19, 256-263.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.8 dialysis against water, pH 6 with several changes of outer fluid inactivates, stable to dialysis for less than 36 h without changing the outer fluid Bos taurus
2.7.4.8 dilution inactivates calf thymus enzyme, KCl protects, not bovine serum albumin Bos taurus
2.7.4.8 KCl does not prevent heat inactivation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.8 dGMP competitive inhibitor to GMP Bos taurus
2.7.4.8 GMP competitive inhibitor to dGMP Bos taurus
2.7.4.8 Li+
-
 Bos taurus
2.7.4.8 Li+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.8 0.08
-
 GMP
-
 Bos taurus
2.7.4.8 0.4
-
 dGMP
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.8 Ca2+ activation Rattus norvegicus
2.7.4.8 Ca2+ activation Bos taurus
2.7.4.8 Co2+
-
 Rattus norvegicus
2.7.4.8 Co2+
-
 Bos taurus
2.7.4.8 Fe2+ no activator Bos taurus
2.7.4.8 Fe3+ no activator Bos taurus
2.7.4.8 K+ activation Rattus norvegicus
2.7.4.8 K+ activation Bos taurus
2.7.4.8 K+ dGMP, not GMP as substrate Rattus norvegicus
2.7.4.8 K+ dGMP, not GMP as substrate Bos taurus
2.7.4.8 Mg2+ requirement Rattus norvegicus
2.7.4.8 Mg2+ requirement Bos taurus
2.7.4.8 Mn2+ requirement Rattus norvegicus
2.7.4.8 Mn2+ requirement Bos taurus
2.7.4.8 additional information
-
 Rattus norvegicus
2.7.4.8 additional information GMP-kinase activity is less sensitive to metal ions than dGMP-kinase activity Bos taurus
2.7.4.8 Na+ activation Bos taurus
2.7.4.8 NH4+ activation Rattus norvegicus
2.7.4.8 NH4+ activation Bos taurus
2.7.4.8 NH4+ less effective than K+ Rattus norvegicus
2.7.4.8 NH4+ less effective than K+ Bos taurus
2.7.4.8 Ni2+ activation Bos taurus
2.7.4.8 Rb+
-
 Rattus norvegicus
2.7.4.8 Rb+
-
 Bos taurus
2.7.4.8 Zn2+
-
 Rattus norvegicus
2.7.4.8 Zn2+
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.8 Bos taurus
-
 calf
-
2.7.4.8 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.8 method that includes DEAE-cellulose, hydroxyapatite and Sephadex G-100 chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.8 bone marrow
-
 Rattus norvegicus
-
2.7.4.8 brain
-
 Rattus norvegicus
-
2.7.4.8 brain
-
 Bos taurus
-
2.7.4.8 kidney
-
 Rattus norvegicus
-
2.7.4.8 liver
-
 Rattus norvegicus
-
2.7.4.8 liver
-
 Bos taurus
-
2.7.4.8 thymus
-
 Rattus norvegicus
-
2.7.4.8 thymus
-
 Bos taurus
-

Storage Stability

EC Number Storage Stability Organism
2.7.4.8 4°C, calf thymus enzyme, stable in the absence of thiols Bos taurus
2.7.4.8 4°C, diluted calf thymus enzyme solution, 0.002 mg/ml, inactivation within 10 days, KCl protects Bos taurus
2.7.4.8 4°C, partially purified calf thymus enzyme preparation in the presence of 1 M KCl, 3 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.8 ATP + dGMP
-
 Rattus norvegicus ADP + dGDP
-
 r
2.7.4.8 ATP + dGMP
-
 Bos taurus ADP + dGDP
-
 r
2.7.4.8 ATP + GMP specificity Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP specificity Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP specificity Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP no donor substrates are ITP, dGTP, dCTP or dTTP Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP no donor substrates are ITP, dGTP, dCTP or dTTP Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP dCMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dCMP is no acceptor substrate Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dCMP is no acceptor substrate Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP dTMP is no acceptor substrate Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dTMP is no acceptor substrate Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP AMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP AMP is no acceptor substrate Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP dAMP is no acceptor substrate Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dAMP is no acceptor substrate Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP dAMP is no acceptor substrate Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP no donor substrates are GTP, CTP, UTP Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP no donor substrates are GTP, CTP, UTP Bos taurus ADP + GDP
-
 r
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Rattus norvegicus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Bos taurus ADP + GDP
-
 ?
2.7.4.8 ATP + GMP CMP, UMP are no acceptor substrates Bos taurus ADP + GDP
-
 r
2.7.4.8 dATP + dGMP
-
 Rattus norvegicus dADP + dGDP
-
 ?
2.7.4.8 dATP + dGMP as good as ATP Bos taurus dADP + dGDP
-
 ?
2.7.4.8 dATP + GMP
-
 Rattus norvegicus dADP + GDP
-
 ?
2.7.4.8 dATP + GMP as good as ATP Bos taurus dADP + GDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.8 100
-
 10 min, about 80% loss of activity, t1/2: 3 min (dGMP-phosphorylation), t1/2: 4 min (GMP-phosphorylation) Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.8 7 10 dGMP-phosphorylation Bos taurus
2.7.4.8 7 10.9 GMP-phosphorylation Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.8 5 10.9 about half-maximal activity at pH 5 and maximal activity at pH 10.9, GMP-phosphorylation, calf thymus Bos taurus
2.7.4.8 5.7 10.9 about half-maximal activity at pH 5.7 and 10.9, dGMP-phosphorylation, calf thymus Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.8 0.064
-
 GMP pH 7.5 Bos taurus
2.7.4.8 0.57
-
 dGMP pH 7.5 Bos taurus