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Literature summary extracted from

  • Ratliff, R.L.; Weaver, R.H.; Lardy, H.A.; Kuby, S.A.
    Nucleoside-triphosphate-nucleoside diphosphate transphosphorylase (nucleoside diphosphokinase). 1.Isolation of the crystalline enzyme from brewers´yeast (1964), J. Biol. Chem., 239, 301-309.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.4.6
-
 Saccharomyces pastorianus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 NDP + NTP Saccharomyces pastorianus
-
 NTP + NDP
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Saccharomyces pastorianus
-
 brewer's yeast, Anheuser-Busch strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6
-
 Saccharomyces pastorianus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.6 1600
-
 25°C Saccharomyces pastorianus

Storage Stability

EC Number Storage Stability Organism
2.7.4.6 -10°C, crude acetone powder, several months Saccharomyces pastorianus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + ADP
-
 Saccharomyces pastorianus ADP + ATP
-
 ?
2.7.4.6 ATP + CDP
-
 Saccharomyces pastorianus ADP + CTP
-
 r
2.7.4.6 ATP + dADP
-
 Saccharomyces pastorianus ADP + dATP
-
 r
2.7.4.6 ATP + dCDP
-
 Saccharomyces pastorianus ADP + dCTP
-
 r
2.7.4.6 ATP + dGDP
-
 Saccharomyces pastorianus ADP + dGTP
-
 r
2.7.4.6 ATP + UDP
-
 Saccharomyces pastorianus ADP + UTP
-
 ?
2.7.4.6 dATP + CDP
-
 Saccharomyces pastorianus dADP + CTP
-
 r
2.7.4.6 dATP + GDP
-
 Saccharomyces pastorianus dADP + GTP
-
 ?
2.7.4.6 dATP + IDP
-
 Saccharomyces pastorianus dADP + ITP
-
 ?
2.7.4.6 dATP + UDP
-
 Saccharomyces pastorianus dADP + UTP
-
 ?
2.7.4.6 GTP + ADP
-
 Saccharomyces pastorianus GDP + ATP
-
 r
2.7.4.6 ITP + ADP
-
 Saccharomyces pastorianus IDP + ATP
-
 ?
2.7.4.6 NDP + NTP
-
 Saccharomyces pastorianus NTP + NDP
-
 r
2.7.4.6 NTP + nucleoside diphosphate broad specificity Saccharomyces pastorianus NDP + NTP
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.6 25
-
 assay at Saccharomyces pastorianus