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Literature summary extracted from

  • Colomb, M.G.; Chéruy, A.; Vignais, P.V.
    Nucleoside diphosphokinase from beef heart cytosol. I. Physical and kinetic properties (1972), Biochemistry, 11, 3370-3378.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.6 unstable in dilute solutions, bovine serum albumin stabilizes, not DTT Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.6 AMP kinetics, substrate inhibition Bos taurus
2.7.4.6 MgADP- in excess Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.6 additional information
-
 additional information effect of Mg2+ on kinetic parameters Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.6 cytosol
-
 Bos taurus 5829
-
2.7.4.6 mitochondrion dual cellular localization in mitochondria and cytosol Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 Mg2+ requirement Bos taurus
2.7.4.6 Mg2+ true substrates: Mg-ADP and Mg-ATP Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.6 103000
-
 sucrose density gradient centrifugation Bos taurus
2.7.4.6 108000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6
-
 Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.4.6 ATP + nucleoside diphosphate = ADP + nucleoside triphosphate mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.6 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.6 360
-
 25°C, pH 8.0 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.4.6 0-2°C, 2 mg bovine serum albumin/ml, 0.04% NaN3, at least 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + adenosine 5'-methylene diphosphonate
-
 Bos taurus ADP + ?
-
 ?
2.7.4.6 ATP + ADP best substrate Bos taurus ADP + ATP
-
 ?
2.7.4.6 ATP + CDP reverse reaction at 21% the rate of ATP plus ADP Bos taurus ADP + CTP
-
 r
2.7.4.6 ATP + UDP reverse reaction at 32% the rate of the reaction ATP plus ADP Bos taurus ADP + UTP
-
 r
2.7.4.6 CTP + CDP reaction at 15% the rate of ATP Bos taurus CDP + CTP
-
 ?
2.7.4.6 GTP + ADP reaction at 12% the rate of ATP Bos taurus GDP + ATP
-
 ?
2.7.4.6 NTP + nucleoside diphosphate the nature of the nucleoside triphosphate controls the rate of the reaction Bos taurus NDP + NTP
-
 ?
2.7.4.6 UTP + UDP reaction at 31% the rate of ATP Bos taurus UDP + UTP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.6 25
-
 assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.6 28
-
 1 h, 0.0018 mg enzyme/ml, in 0.1 M phosphate buffer, pH 7, more than 50% loss of activity, 2 mg/ml bovine serum albumin plus 2 mM Mg-ADP or 5 mM free ADP protect, not MgCl2 or free ADP alone Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.6 5
-
 inactivation below Bos taurus