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Literature summary extracted from

  • Voglmaier, S.M.; Bembenek, M.E.; Kaplin, A.I.; Dorman, G.; Olszewski, J.D.; Prestwich, G.D.; Snyder, S.H.
    Purified inositol hexakisphosphate kinase is an ATP synthase: diphosphoinositol pentakisphosphate as a high-energy phosphate donor (1996), Proc. Natl. Acad. Sci. USA, 93, 4305-4310.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.21 1,3,4,5,6-inositol pentakisphosphate IC50 for inositol hexakisphosphate kinase activity is 0.022 mM, IC50 for ATP synthase activity is 0.0128 mM Rattus norvegicus
2.7.4.21 diphosphoinositol pentakisphosphate IC50 for inositol hexakisphosphate kinase activity is 0.0066 mM, IC50 for ATP synthase activity is 0.0018 mM Rattus norvegicus
2.7.4.21 inositol 1,2,4,5,6-pentakisphosphate IC50 for inositol hexakisphosphate kinase activity is 0.022 mM Rattus norvegicus
2.7.4.21 Inositol 1,3,4,5-tetrakisphosphate IC50 for inositol hexakisphosphate kinase activity is 0.061 mM, IC50 for ATP synthase activity is 0.0325 mM Rattus norvegicus
2.7.4.21 Inositol 1,4,5-trisphosphate IC50 for ATP synthase activity is 0.253 mM Rattus norvegicus
2.7.4.21 Inositol hexakisphosphate IC50 for inositol hexakisphosphate kinase activity is 0.0008 mM, IC50 for ATP synthase activity is 0.0018 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.21 0.0007
-
 Inositol hexakisphosphate pH 6.8, 37°C Rattus norvegicus
2.7.4.21 0.00197
-
 5-diphospho-1D-myo-inositol (1,2,3,4,6)pentakisphosphate pH 6.8, 37°C Rattus norvegicus
2.7.4.21 1.35
-
 ATP pH 6.8, 37°C Rattus norvegicus
2.7.4.21 1.57
-
 ADP pH 6.8, 37°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.21 54000
-
 1 * 54000, SDS-PAGE Rattus norvegicus
2.7.4.21 60000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.21 ATP + 1D-myo-inositol hexakisphosphate Rattus norvegicus enzyme is responsible for the biosynthesis of diphospho-myo-inositol pentakisphosphate. The enzyme also has a ATP synthase activity, implying that 5-diphospho-1D-myo-inositol pentakisphosphate functions as high-energy phosphate donor ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
 r
2.7.4.24 ATP + 5-diphospho-1D-myo-inositol-pentakisphosphate Rattus norvegicus
-
 ADP + bis(diphospho)-1D-myo-inositol tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.21 Rattus norvegicus
-
-
-
2.7.4.24 Rattus norvegicus
-
 male Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.21
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.21 brain
-
 Rattus norvegicus
-
2.7.4.21 heart weak activity Rattus norvegicus
-
2.7.4.21 kidney weak Rattus norvegicus
-
2.7.4.21 liver weak activity Rattus norvegicus
-
2.7.4.21 thymus
-
 Rattus norvegicus
-
2.7.4.24 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.21 1.558
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.4.21 -70°C, 20% glycerol, optimal stability Rattus norvegicus
2.7.4.21 4°C, 7 days, 50% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.21 ATP + 1D-myo-inositol hexakisphosphate forward and reverse reaction are random bireactant systems Rattus norvegicus ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
 ?
2.7.4.21 ATP + 1D-myo-inositol hexakisphosphate forward and reverse reaction are random bireactant systems Rattus norvegicus ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
 r
2.7.4.21 ATP + 1D-myo-inositol hexakisphosphate enzyme is responsible for the biosynthesis of diphospho-myo-inositol pentakisphosphate. The enzyme also has a ATP synthase activity, implying that 5-diphospho-1D-myo-inositol pentakisphosphate functions as high-energy phosphate donor Rattus norvegicus ADP + 5-diphospho-1D-myo-inositol 1,2,3,4,6-pentakisphosphate
-
 r
2.7.4.24 ATP + 5-diphospho-1D-myo-inositol-pentakisphosphate
-
 Rattus norvegicus ADP + bis(diphospho)-1D-myo-inositol tetrakisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.4.21 monomer 1 * 54000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.21 6.8
-
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.7.4.21 0.0018
-
 IC50 for inositol hexakisphosphate kinase activity is 0.0008 mM, IC50 for ATP synthase activity is 0.0018 mM Rattus norvegicus Inositol hexakisphosphate
2.7.4.21 0.0018
-
 IC50 for inositol hexakisphosphate kinase activity is 0.0066 mM, IC50 for ATP synthase activity is 0.0018 mM Rattus norvegicus diphosphoinositol pentakisphosphate
2.7.4.21 0.0128
-
 IC50 for inositol hexakisphosphate kinase activity is 0.022 mM, IC50 for ATP synthase activity is 0.0128 mM Rattus norvegicus 1,3,4,5,6-inositol pentakisphosphate
2.7.4.21 0.022
-
 IC50 for inositol hexakisphosphate kinase activity is 0.022 mM Rattus norvegicus inositol 1,2,4,5,6-pentakisphosphate
2.7.4.21 0.0325
-
 IC50 for inositol hexakisphosphate kinase activity is 0.061 mM, IC50 for ATP synthase activity is 0.0325 mM Rattus norvegicus His3Cd-GST-protein
2.7.4.21 0.253
-
 IC50 for ATP synthase activity is 0.253 mM Rattus norvegicus human skin elastin