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Literature summary extracted from

  • Bello, L.J.; Bessman, M.J.
    The enzymology of virus-infected bacteria, IV. Purification and properties of the deoxynucleotide kinase induced by bacteriophage T2 (1963), J. Biol. Chem., 238, 1777-1787.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.12 5-hydroxymethyl-dCMP dGMP or dTMP as substrate Escherichia coli
2.7.4.12 dGMP dTMP or 5-hydroxymethyl-dCMP as substrate Escherichia coli
2.7.4.12 dTMP dGMP or 5-hydroxymethyl-dCMP as substrate Escherichia coli
2.7.4.12 additional information tryptic digestion inactivates Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.12 0.056
-
 5-hydroxymethyl-dCMP pH 7.4, 37°C Escherichia coli
2.7.4.12 0.085
-
 dGMP pH 7.4, 37°C Escherichia coli
2.7.4.12 0.278 0.313 dTMP pH 7.4, 37°C Escherichia coli
2.7.4.12 0.82
-
 ATP pH 7.4, 37°C, with dTMP Escherichia coli
2.7.4.12 1.25
-
 ATP pH 7.4, 37°C, with dGMP Escherichia coli
2.7.4.12 5
-
 ATP pH 7.4, 37°C, with 5-hydroxymethyl-dCMP Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.12 Ca2+ requirement, about 60% as effective as Mn2+, dGMP as substrate Escherichia coli
2.7.4.12 Mg2+ requirement, about 90% as effective as Mn2+, dGMP as substrate Escherichia coli
2.7.4.12 Mn2+ requirement, dGMP as substrate Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.12 ATP + dGMP Escherichia coli or dTMP ADP + dGDP
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.12 Escherichia coli
-
 infected with bacteriophage T2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.12 infected with bacteriophage T2 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.12 24.16
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.12 -10°C, crude preparation, at least a year Escherichia coli
2.7.4.12 4°C, partially purified preparation, at least 2 months Escherichia coli
2.7.4.12 at -10°C to 4°C, 1 mM EDTA, up to 20% loss of activity within 6 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.12 ATP + 5-hydroxymethyl-dCMP phosphorylation at about half the rate of dGMP or dTMP Escherichia coli ADP + 5-hydroxymethyl-dCDP
-
 r
2.7.4.12 ATP + dGMP as good as dTMP Escherichia coli ADP + dGDP
-
 r
2.7.4.12 ATP + dGMP or dTMP Escherichia coli ADP + dGDP
-
 r
2.7.4.12 ATP + dTMP as good as dGMP Escherichia coli ADP + dTDP
-
 r
2.7.4.12 dATP + dGMP phosphorylation at about 80-90% the rate of ATP Escherichia coli dADP + dGDP
-
 r
2.7.4.12 dATP + dTMP phosphorylation at about 80-90% the rate of ATP Escherichia coli dADP + dTDP
-
 r
2.7.4.12 additional information poor substrates are dUMP or 5-methyl-dCMP, no donor substrates are GTP, CTP, dGTP, dCTP, or dTTP, no acceptor substrates are dCMP, dAMP, GMP, or UMP Escherichia coli ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.12 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.12 8
-
 dTMP or dGMP Escherichia coli
2.7.4.12 8.6
-
 5-hydroxymethyl-dCMP Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.12 7 9.3 pH 7: about 75% of maximal activity of 5-hydroxymethyl-dCMP, 84% of maximal activity dTMP or 87% of maximal activity of dGMP, pH 9.3: about 79% of maximal activity of dTMP, 83% of maximal activity of dGMP or 90% of maximal activity of 5-hydroxymethyl-dCMP Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.4.12 4
-
 3 min, about 60% loss of activity at 37°C Escherichia coli
2.7.4.12 4.4
-
 37°C, 3 min, about 30% loss of activity, and about 45% loss of activity within 6 min Escherichia coli
2.7.4.12 4.7
-
 15 min, about 20% loss of activity at 37°C Escherichia coli
2.7.4.12 5.4
-
 15 min stable at 37°C Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.12 0.04
-
 5-hydroxymethyl-dCMP pH 7.4, 37°C, dTMP is substrate Escherichia coli
2.7.4.12 0.044
-
 5-hydroxymethyl-dCMP pH 7.4, 37°C, dGMP is substrate Escherichia coli
2.7.4.12 0.064
-
 dGMP pH 7.4, 37°C, dTMP is substrate Escherichia coli
2.7.4.12 0.25
-
 dTMP pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate Escherichia coli
2.7.4.12 0.314
-
 dTMP pH 7.4, 37°C, dGMP is substrate Escherichia coli
2.7.4.12 1.28
-
 dGMP pH 7.4, 37°C, 5-hydroxymethyl-dCMP is substrate Escherichia coli