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Literature summary extracted from

  • Keng, Y.F.; Viola, R.E.
    Specificity of aspartokinase III from Escherichia coli and an examination of important catalytic residues (1996), Arch. Biochem. Biophys., 335, 73-81.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 lysC geneencoding for AKIII cloned into vector pTZ19U and expressed in the protease-deficient Escherichia coli BL21DE3 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 D-aspartate beta-hydroxamate
-
 Escherichia coli
2.7.2.4 D-aspartic acid 1-benzyl ester
-
 Escherichia coli
2.7.2.4 Diethyl aminomalonate
-
 Escherichia coli
2.7.2.4 diethyldicarbonate
-
 Escherichia coli
2.7.2.4 DL-3-amino-4-hydroxybutyrate methyl ester
-
 Escherichia coli
2.7.2.4 DL-3-aminobutyrate
-
 Escherichia coli
2.7.2.4 L-2-aminobutyrate competitive inhibitor Escherichia coli
2.7.2.4 L-glutamate
-
 Escherichia coli
2.7.2.4 L-glutamate gamma-methyl ester
-
 Escherichia coli
2.7.2.4 L-homoserine
-
 Escherichia coli
2.7.2.4 L-malate
-
 Escherichia coli
2.7.2.4 L-norvaline
-
 Escherichia coli
2.7.2.4 malonate
-
 Escherichia coli
2.7.2.4 N-Acetylimidazole
-
 Escherichia coli
2.7.2.4 N-ethylmaleimide
-
 Escherichia coli
2.7.2.4 succinate
-
 Escherichia coli
2.7.2.4 Tetranitromethane
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 0.6
-
 L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 2.5
-
 L-aspartate beta-benzyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 4.9
-
 L-aspartate beta-methyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 5.3
-
 L-aspartic acid 1-benzyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 13
-
 L-aspartate beta-hydroxamate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 16
-
 L-asparagine pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 41
-
 N-formyl-L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 48
-
 N-acetyl-L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 68
-
 N-Chloroacetyl-L-aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 147
-
 DL-threo-3-methyl aspartate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 184
-
 L-aspartic acid amide pH 8.0, 25°C, aspartokinase III Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 50000
-
 2 * 50000 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 aspartokinase III Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + DL-threo-3-methyl aspartate aspartokinase III Escherichia coli ADP + 3-methyl-4-phosphoaspartate
-
 ?
2.7.2.4 ATP + L-asparagine aspartokinase III Escherichia coli ?
-
 ?
2.7.2.4 ATP + L-aspartate beta-hydroxamate aspartokinase III Escherichia coli ?
-
 ?
2.7.2.4 ATP + L-aspartate beta-methyl ester aspartokinase III Escherichia coli ?
-
 ?
2.7.2.4 ATP + L-aspartic acid 1-benzyl ester aspartokinase III Escherichia coli ?
-
 ?
2.7.2.4 ATP + L-aspartic acid 4-benzyl ester aspartokinase III Escherichia coli ?
-
 ?
2.7.2.4 ATP + L-aspartic acid amide aspartokinase III Escherichia coli ADP + 4-phospho-L-aspartic acid amide
-
 ?
2.7.2.4 ATP + N-acetyl-L-aspartate aspartokinase III Escherichia coli ADP + N-acetyl-4-phospho-L-aspartate
-
 ?
2.7.2.4 ATP + N-chloroacetyl-L-aspartate aspartokinase III Escherichia coli ADP + N-chloroacetyl-4-phospho-L-aspartate
-
 ?
2.7.2.4 ATP + N-formyl-L-aspartate aspartokinase III Escherichia coli ADP + N-formyl-4-phospho-L-aspartate
-
 ?
2.7.2.4 additional information aspartokinase III, D-isomers of the derivatives of aspartic acid, including D-aspartate alpha-benzyl ester and D-aspartate beta-hydroxamate are not substrates regardless of whether the alpha- or the beta-carboxyl group is derivatized, L-cysteine sulfinate and 2-methyl-DL-aspartate are no substrates Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 dimer 2 * 50000 Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.2.4 aspartokinase III
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.4 7 9
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.4 6 11
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.4 7.7
-
 L-homoserine pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 14
-
 D-aspartic acid 1-benzyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 14
-
 DL-3-amino-4-hydroxybutyrate methyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 28
-
 L-2-aminobutyrate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 32
-
 L-norvaline pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 53
-
 L-malate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 63
-
 Diethyl aminomalonate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 82
-
 L-glutamate gamma-methyl ester pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 87
-
 D-aspartate beta-hydroxamate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 108
-
 malonate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 128
-
 L-glutamate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 159
-
 succinate pH 8.0, 25°C, aspartokinase III Escherichia coli
2.7.2.4 245
-
 DL-3-aminobutyrate pH 8.0, 25°C, aspartokinase III Escherichia coli