Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dotson, S.B.; Somers, D.A.; Gengenbach, B.G.
    Purification and characterization of lysine-sensitive aspartate kinase from maize cell cultures (1989), Plant Physiol., 91, 1602-1608.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-lysine
-
Zea mays
2.7.2.4 L-Lysine ethyl ester
-
Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.2.4 chloroplast
-
Zea mays 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 49000
-
2 * 49000 + 2 * 60000, SDS-PAGE Zea mays
2.7.2.4 60000
-
2 * 49000 + 2 * 60000, SDS-PAGE Zea mays
2.7.2.4 113000
-
gel filtration, native PAGE, isoenzyme AK Late Zea mays
2.7.2.4 255000
-
gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Zea mays first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine ADP + 4-phospho-L-aspartate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Zea mays
-
maize
-
2.7.2.4 Zea mays
-
Black Mexican Sweet
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.4 cell suspension culture
-
Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.4 additional information
-
18.9 units/mg Zea mays

Storage Stability

EC Number Storage Stability Organism
2.7.2.4 -70°C, stored in buffer containing 200 mM KCl and 2 mg/ml bovine serum albumin, stable for more than 1 year Zea mays
2.7.2.4 23°C, more than 90% stable for up to 1 week Zea mays
2.7.2.4 4°C, stable for more than 1 week Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
Zea mays ADP + 4-phospho-L-aspartate
-
r
2.7.2.4 ATP + L-aspartate first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine Zea mays ADP + 4-phospho-L-aspartate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.2.4 tetramer native AK Late isoform is a tetramer which dissociates into active dimers during native gradient PAGE Zea mays
2.7.2.4 tetramer 2 * 49000 + 2 * 60000, SDS-PAGE Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.4 8
-
-
Zea mays