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Literature summary extracted from

  • Dungan, S.M.; Datta, P.
    Concerted feedback inhibition. Purification and some properties of aspartokinase from Pseudomonas fluorescens (1973), J. Biol. Chem., 248, 8534-8540.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.4 L-lysine slightly stimulatory Pseudomonas fluorescens
2.7.2.4 L-methionine slightly stimulatory Pseudomonas fluorescens
2.7.2.4 L-threonine slight activation at low concentration Pseudomonas fluorescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-lysine concerted feedback inhibition with L-threonine Pseudomonas fluorescens
2.7.2.4 L-threonine above 10 mM Pseudomonas fluorescens
2.7.2.4 L-Threonine methyl ester in combination with either L-lysine or L-methionine Pseudomonas fluorescens
2.7.2.4 methionine concerted feedback inhibition with L-threonine Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 K+ addition of 200 mM increases the reaction rate by about 40% Pseudomonas fluorescens
2.7.2.4 Mn2+
-
 Pseudomonas fluorescens
2.7.2.4 additional information Li+ and Na+ ions have no effect, Zn2+, Ca2+ and Co2+ cannot replace Mg2+ or Mn2+ Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.4 43000
-
 3 * 43000, SDS-PAGE Pseudomonas fluorescens
2.7.2.4 133000
-
 sedimentation velocity centrifugation Pseudomonas fluorescens
2.7.2.4 137000
-
 gel filtration Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Pseudomonas fluorescens first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine ADP + 4-phospho-L-aspartate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Pseudomonas fluorescens
-
 wild-type strain P-14
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4
-
 Pseudomonas fluorescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.4 11.16
-
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Pseudomonas fluorescens ADP + 4-phospho-L-aspartate
-
 r
2.7.2.4 ATP + L-aspartate first step common to the biosynthesis of L-lysine, L-threonine, isoleucine and methionine Pseudomonas fluorescens ADP + 4-phospho-L-aspartate
-
 r
2.7.2.4 additional information absolute requirement for ATP, no other nucleoside phosphates serve as phosphate donor, analogs of aspartate such as L-glutamate, DL-alpha-methyl aspartate, N-acetyl aspartate and D-aspartate are inactive as acceptors Pseudomonas fluorescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.4 trimer 3 * 43000, SDS-PAGE Pseudomonas fluorescens