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Literature summary extracted from

  • Manca de Nadra, M.C.; Nadra Chaud, C.A.; Pesce de Ruiz Holgado, A.; Oliver, G.
    Carbamate kinase of Lactobacillus buchneri NCDO110. I. Purification and properties (1986), Biotechnol. Appl. Biochem., 8, 46-52.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.2 AgNO3
-
 Lentilactobacillus buchneri
2.7.2.2 ATP
-
 Lentilactobacillus buchneri
2.7.2.2 CTP
-
 Lentilactobacillus buchneri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.2 0.71
-
 ADP 30°C, pH 5.0 Lentilactobacillus buchneri
2.7.2.2 0.71
-
 ADP ADP in form of MgADP- Lentilactobacillus buchneri
2.7.2.2 1.17
-
 ADP 30°C, pH 5.0 Lentilactobacillus buchneri
2.7.2.2 1.17
-
 ADP ADP in form of MnADP- Lentilactobacillus buchneri
2.7.2.2 1.53
-
 Carbamoyl phosphate cosubstrate MnADP-, 30°C, pH 5.0 Lentilactobacillus buchneri
2.7.2.2 1.63
-
 Carbamoyl phosphate cosubstrate MgADP-, 30°C, pH 5.0 Lentilactobacillus buchneri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.2 Mg2+ Mn2+ or Mg2+ required, higher activity with Mn2+ Lentilactobacillus buchneri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.2 97000
-
 gel electrophoresis Lentilactobacillus buchneri

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Lentilactobacillus buchneri
-
 NCDO110
-
2.7.2.2 Lentilactobacillus buchneri NCDO110
-
 NCDO110
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.2
-
 Lentilactobacillus buchneri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.2 59.52
-
-
 Lentilactobacillus buchneri

Storage Stability

EC Number Storage Stability Organism
2.7.2.2 -20°C, 2 weeks, stable Lentilactobacillus buchneri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
 Lentilactobacillus buchneri ATP + carbamate
-
 ?
2.7.2.2 ADP + carbamoyl phosphate
-
 Lentilactobacillus buchneri NCDO110 ATP + carbamate
-
 ?
2.7.2.2 ATP + NH3 + CO2
-
 Lentilactobacillus buchneri ADP + carbamoyl phosphate
-
 ?
2.7.2.2 ATP + NH3 + CO2
-
 Lentilactobacillus buchneri NCDO110 ADP + carbamoyl phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.2 30
-
-
 Lentilactobacillus buchneri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.2 5.4 8 30°C, pH 5.0 Lentilactobacillus buchneri

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.2 4.4
-
 CTP plus Mg2+, 30°C, pH 5.0 Lentilactobacillus buchneri
2.7.2.2 6.2
-
 ATP plus Mn2+, 30°C, pH 5.0 Lentilactobacillus buchneri
2.7.2.2 9.4
-
 ATP plus Mg2+, 30°C, pH 5.0 Lentilactobacillus buchneri