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Literature summary extracted from

  • Miles, R.D.; Iyer, P.P.; Ferry, J.G.
    Site-directed mutational analysis of active site residues in the acetate kinase from Methanosarcina thermophila (2001), J. Biol. Chem., 276, 45059-45064.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 cloned and hyper-expressed in Escherichia coli Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.1 D148A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 D148E site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 D148N site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 E384A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 K14A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 K14R site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 N211A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 N7A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 S10A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 S11A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 S12A site-directed mutagenesis Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 1.5
-
 ATP
-
 Methanosarcina thermophila
2.7.2.1 2.13
-
 ATP pH 7.4, 37°C, wild-type Methanosarcina thermophila
2.7.2.1 3
-
 ATP pH 7.4, 37°C, mutant N211A Methanosarcina thermophila
2.7.2.1 3.1
-
 ATP pH 7.4, 37°C, mutant D148A Methanosarcina thermophila
2.7.2.1 3.2
-
 ATP pH 7.4, 37°C, mutant S10A Methanosarcina thermophila
2.7.2.1 3.62
-
 ATP pH 7.4, 37°C, mutant K14R Methanosarcina thermophila
2.7.2.1 3.7
-
 ATP pH 7.4, 37°C, mutant S12T Methanosarcina thermophila
2.7.2.1 4.3
-
 ATP pH 7.4, 37°C, mutant S10T Methanosarcina thermophila
2.7.2.1 4.5
-
 ATP pH 7.4, 37°C, mutant S11A Methanosarcina thermophila
2.7.2.1 5
-
 ATP pH 7.4, 37°C, mutant N7D, D148E Methanosarcina thermophila
2.7.2.1 5.2
-
 ATP pH 7.4, 37°C, mutant K14A Methanosarcina thermophila
2.7.2.1 5.8
-
 ATP pH 7.4, 37°C, mutant S10T Methanosarcina thermophila
2.7.2.1 6.8
-
 ATP pH 7.4, 37°C, mutant S11T Methanosarcina thermophila
2.7.2.1 7
-
 ATP pH 7.4, 37°C, mutant N7A Methanosarcina thermophila
2.7.2.1 7.3
-
 ATP pH 7.4, 37°C, mutant S12A Methanosarcina thermophila
2.7.2.1 7.7
-
 ATP pH 7.4, 37°C, mutant E384A Methanosarcina thermophila
2.7.2.1 48
-
 acetate pH 7.4, 37°C, wild-type Methanosarcina thermophila
2.7.2.1 66
-
 acetate
-
 Methanosarcina thermophila
2.7.2.1 111
-
 acetate pH 7.4, 37°C, mutant N211A Methanosarcina thermophila
2.7.2.1 170
-
 acetate pH 7.4, 37°C, mutant D148A Methanosarcina thermophila
2.7.2.1 200
-
 acetate pH 7.4, 37°C, mutant S11A Methanosarcina thermophila
2.7.2.1 239
-
 acetate pH 7.4, 37°C, mutant K14R Methanosarcina thermophila
2.7.2.1 266
-
 acetate pH 7.4, 37°C, mutant S10T Methanosarcina thermophila
2.7.2.1 333
-
 acetate pH 7.4, 37°C, mutant S12T Methanosarcina thermophila
2.7.2.1 390
-
 acetate pH 7.4, 37°C, mutant D148N Methanosarcina thermophila
2.7.2.1 693
-
 acetate pH 7.4, 37°C, mutant N7D Methanosarcina thermophila
2.7.2.1 814
-
 acetate pH 7.4, 37°C, mutant S10A Methanosarcina thermophila
2.7.2.1 1573
-
 acetate pH 7.4, 37°C, mutant S11T Methanosarcina thermophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+
-
 Methanosarcina thermophila
2.7.2.1 Mg2+
-
 Methanosarcina thermophila
2.7.2.1 Mn2+
-
 Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Methanosarcina thermophila
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 0.28
-
 mutant D148A Methanosarcina thermophila
2.7.2.1 3.4
-
 mutant E384A Methanosarcina thermophila
2.7.2.1 9
-
 mutant S10A Methanosarcina thermophila
2.7.2.1 13
-
 mutant N7A Methanosarcina thermophila
2.7.2.1 19
-
 mutant S12A Methanosarcina thermophila
2.7.2.1 102
-
 mutant K14A Methanosarcina thermophila
2.7.2.1 324
-
 mutant S11A Methanosarcina thermophila
2.7.2.1 539
-
 mutant N211A Methanosarcina thermophila
2.7.2.1 584
-
 wild-type Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r