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Literature summary extracted from

  • Singh-Wissmann, K.; Miles, R.D.; Ingram-Smith, C.; Ferry, J.G.
    Identification of essential arginines in the acetate kinase from Methanosarcina thermophila (2000), Biochemistry, 39, 3671-3677.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15b Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.1 R175K site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 R285A site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 R285K site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 R285L site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 R340K site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 R340L site-directed mutagenesis Methanosarcina thermophila
2.7.2.1 R91K site-directed mutagenesis Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 Phenylglyoxal
-
 Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.9
-
 ATP pH 7.6, 37°C, mutant R340L Methanosarcina thermophila
2.7.2.1 1
-
 ATP pH 7.6, 37°C, wild-type and mutant R175K Methanosarcina thermophila
2.7.2.1 1.3
-
 ATP pH 7.6, 37°C, mutant R340K Methanosarcina thermophila
2.7.2.1 1.4
-
 ATP
-
 Methanosarcina thermophila
2.7.2.1 2.3
-
 acetate
-
 Methanosarcina thermophila
2.7.2.1 2.8
-
 ATP pH 7.6, 37°C, recombinant wild-type Methanosarcina thermophila
2.7.2.1 3
-
 ATP pH 7.6, 37°C, mutant R285K, R285L Methanosarcina thermophila
2.7.2.1 3.4
-
 ATP pH 7.6, 37°C, mutant R241K Methanosarcina thermophila
2.7.2.1 3.9
-
 ATP pH 7.6, 37°C, mutant R91K Methanosarcina thermophila
2.7.2.1 4
-
 acetate pH 7.6, 37°C, mutant R175K Methanosarcina thermophila
2.7.2.1 6
-
 ATP pH 7.6, 37°C, mutant R285A, R91A Methanosarcina thermophila
2.7.2.1 7.2
-
 acetate
-
 Methanosarcina thermophila
2.7.2.1 19
-
 acetate pH 7.6, 37°C Methanosarcina thermophila
2.7.2.1 20
-
 acetate pH 7.6, 37°C, mutant R340L Methanosarcina thermophila
2.7.2.1 42
-
 acetate pH 7.6, 37°C, mutant R340K Methanosarcina thermophila
2.7.2.1 129
-
 acetate pH 7.6, 37°C, mutant R285L Methanosarcina thermophila
2.7.2.1 206
-
 acetate pH 7.6, 37°C, mutant R285A Methanosarcina thermophila
2.7.2.1 219
-
 acetate pH 7.6, 37°C, mutant R285K Methanosarcina thermophila
2.7.2.1 260
-
 acetate pH 7.6, 37°C, mutant R91K Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Methanosarcina thermophila
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 purified wild-type and recombinant enzymes, R175L and R340A are unstable and cannot be purified Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r