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Literature summary extracted from

  • Winzer, K.; Lorenz, K.; Duerre, P.
    Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesis (1997), Microbiology, 143, 3279-3286.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.1 DNA fragment encoding 246 bp of the acetate kinase gene of strain DSM 792 cloned by a PCR-based approach Clostridium acetobutylicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 HgCl2
-
 Clostridium acetobutylicum
2.7.2.1 p-hydroxymercuribenzoate
-
 Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.37
-
 ATP pH 7.3, 30°C Clostridium acetobutylicum
2.7.2.1 0.58
-
 acetyl phosphate pH 7.3, 30°C Clostridium acetobutylicum
2.7.2.1 0.71
-
 ADP pH 7.3, 30°C Clostridium acetobutylicum
2.7.2.1 73
-
 acetate pH 7.3, 30°C Clostridium acetobutylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+
-
 Clostridium acetobutylicum
2.7.2.1 Co2+
-
 Clostridium acetobutylicum
2.7.2.1 Mn2+
-
 Clostridium acetobutylicum
2.7.2.1 additional information no activity with Zn2+ Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 43000
-
 2 * 43000, SDS-PAGE Clostridium acetobutylicum
2.7.2.1 87000 94000 gel filtration, native gel electrophoresis Clostridium acetobutylicum
2.7.2.1 89000
-
 native PAGE Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Clostridium acetobutylicum
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Clostridium acetobutylicum
-
 DSM 1731 and DSM 792
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 1087
-
-
 Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Clostridium acetobutylicum ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + propionate
-
 Clostridium acetobutylicum ADP + propionyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Clostridium acetobutylicum CDP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Clostridium acetobutylicum GDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Clostridium acetobutylicum IDP + acetyl phosphate
-
 r
2.7.2.1 additional information formate and butyrate are no substrates Clostridium acetobutylicum ?
-
 ?
2.7.2.1 UTP + acetate
-
 Clostridium acetobutylicum UDP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.1 dimer 2 * 43000, SDS-PAGE Clostridium acetobutylicum