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Literature summary extracted from

  • Diez-Gonzalez, F.; Russell, J.B.; Hunter, J.B.
    The acetate kinase of Clostridium acetobutylicum strain P262 (1996), Arch. Microbiol., 166, 418-420.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 additional information iodoacetate and iodoacetamide does not inhibit Clostridium acetobutylicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 2.5
-
 ATP pH 7.0, 36°C Clostridium acetobutylicum
2.7.2.1 6
-
 ADP pH 7.0, 36°C Clostridium acetobutylicum
2.7.2.1 160
-
 acetate pH 7.0, 36°C Clostridium acetobutylicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+ strict requirement Clostridium acetobutylicum
2.7.2.1 Mn2+
-
 Clostridium acetobutylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 42000
-
 2 * 42000, SDS-PAGE Clostridium acetobutylicum
2.7.2.1 78000
-
 gel filtration Clostridium acetobutylicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Clostridium acetobutylicum
-
 P262
-
2.7.2.1 Clostridium acetobutylicum P262
-
 P262
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Clostridium acetobutylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 27.7
-
-
 Clostridium acetobutylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + propionate 40% lower kinase activity than acetate Clostridium acetobutylicum ADP + propionyl phosphate
-
 r
2.7.2.1 ATP + propionate 40% lower kinase activity than acetate Clostridium acetobutylicum P262 ADP + propionyl phosphate
-
 r
2.7.2.1 additional information butyrate is no substrate Clostridium acetobutylicum ?
-
 ?
2.7.2.1 additional information butyrate is no substrate Clostridium acetobutylicum P262 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.1 dimer 2 * 42000, SDS-PAGE Clostridium acetobutylicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 238
-
 ATP pH 7.0, 36°C Clostridium acetobutylicum
2.7.2.1 280
-
 acetate pH 7.0, 36°C Clostridium acetobutylicum
2.7.2.1 532
-
 ADP pH 7.0, 36°C Clostridium acetobutylicum
2.7.2.1 700
-
 acetyl phosphate pH 7.0, 36°C Clostridium acetobutylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 8
-
 30% activity still observed at pH 5.5 Clostridium acetobutylicum