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Literature summary extracted from

  • Shim, Y.H.; Lin, C.H.; Strickland, K.P.
    The purification and properties of monoacylglycerol kinase from bovine brain (1989), Biochem. Cell Biol., 67, 233-241.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.94 additional information protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes Bos taurus
2.7.1.94 Phospholipids e.g. phosphatidylcholine, phosphatidylethanolamine or sphingomyelin, inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus

General Stability

EC Number General Stability Organism
2.7.1.94 DTT, EDTA or ATP stabilizes during purification Bos taurus
2.7.1.94 protein peak eluted from hydroxylapatite by 25 mM phosphate activates and stabilizes Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.94 5,5'-dithiobis(2-nitrobenzoic acid) DTT prevents inhibition Bos taurus
2.7.1.94 NEM DTT prevents inhibition Bos taurus
2.7.1.94 p-chloromercuriphenylsulfonate DTT prevents inhibition Bos taurus
2.7.1.94 phosphatidylcholine inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus
2.7.1.94 phosphatidylethanolamine inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus
2.7.1.94 sphingomyelin inhibition alone, synergistic activation with the protein peak eluted from hydroxylapatite by 25 mM phosphate Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.94 cytosol
-
Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.94 Ca2+ 5 mM, 58% as effective as Mg2+ in activation Bos taurus
2.7.1.94 Co2+ 5 mM, 60% as effective as Mg2+ in activation Bos taurus
2.7.1.94 Cu2+ 5 mM, 51% as effective as Mg2+ in activation Bos taurus
2.7.1.94 Fe2+ 5 mM, 60% as effective as Mg2+ in activation Bos taurus
2.7.1.94 Mg2+ required Bos taurus
2.7.1.94 Mg2+ optimal concentration is 10 mM Bos taurus
2.7.1.94 Mn2+ 5 mM, as effective as Mg2+ in activation Bos taurus
2.7.1.94 Ni2+ 5 mM, 60% as effective as Mg2+ in activation Bos taurus
2.7.1.94 Zn2+ 5 mM, 48% as effective as Mg2+ in activation Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.94 72000
-
x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.94 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.94
-
Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.94 brain
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.94 additional information
-
-
Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.94 ATP + 1-arachidonoylglycerol
-
Bos taurus ADP + 1-arachidonoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-arachidoylglycerol
-
Bos taurus ADP + 1-arachidoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-caproylglycerol
-
Bos taurus ADP + 1-caproylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-capryloylglycerol
-
Bos taurus ADP + 1-capryloylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-lauroylglycerol
-
Bos taurus ADP + 1-lauroylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-linolenoylglycerol
-
Bos taurus ADP + 1-linolenoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-linoleoylglycerol
-
Bos taurus ADP + 1-linoleoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-monoolein i.e. 1-monooleolylglyerol Bos taurus ADP + 1-oleoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-myristoylglycerol
-
Bos taurus ADP + 1-myristoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-palmitoleoylglycerol
-
Bos taurus ADP + 1-palmitoleoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-palmitoylglycerol
-
Bos taurus ADP + 1-palmitoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 1-stearoylglycerol
-
Bos taurus ADP + 1-stearoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-arachidonoylglycerol
-
Bos taurus ADP + 2-arachidonoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-arachidoylglycerol
-
Bos taurus ADP + 2-arachidoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-caproylglycerol
-
Bos taurus ADP + 2-caproylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-lauroylglycerol
-
Bos taurus ADP + 2-lauroylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-linolenoylglycerol
-
Bos taurus ADP + 2-linolenoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-linoleoylglycerol
-
Bos taurus ADP + 2-linoleoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-monoolein i.e. 2-monooleolylglyerol Bos taurus ADP + 2-oleoyoglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-palmitoleoylglycerol
-
Bos taurus ADP + 2-palmitoleoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-palmitoylglycerol
-
Bos taurus ADP + 2-palmitoylglycerol 3-phosphate
-
?
2.7.1.94 ATP + 2-stearoylglycerol
-
Bos taurus ADP + 2-stearoylglycerol 3-phosphate
-
?
2.7.1.94 additional information 1-monostearin amongst the 1-acylglycerols and 2-monoarachidonin amongst the 2-acylglycerols give the highest activity Bos taurus ?
-
?
2.7.1.94 additional information preference for substrates with unsaturated fatty acids except for 1- and 2-monostearins Bos taurus ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.94 ? x * 72000, SDS-PAGE, enzyme exists as a complex that is stabilized by 0.5 M NaCl and, on complete dissociation yields a major protein of MW 72000 Bos taurus