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Literature summary extracted from

  • Ding, Y.H.; Ronimus, R.S.; Morgan, H.W.
    Purification and properties of the pyrophosphate-dependent phosphofructokinase from Dictyoglomus thermophilum Rt46 B.1 (1999), Extremophiles, 3, 131-137.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 Cu2+ complete inhibition at 1 mM Dictyoglomus thermophilum
2.7.1.90 Fe3+ 84% inhibition at 0.1 mM Dictyoglomus thermophilum
2.7.1.90 Mn2+ 46% inhibition at 0.1 mM Dictyoglomus thermophilum
2.7.1.90 Se4+ 75% inhibition at 0.1 mM Dictyoglomus thermophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.022
-
 diphosphate pH 6.0, 50°C Dictyoglomus thermophilum
2.7.1.90 0.23
-
 D-fructose 6-phosphate pH 6.0, 50°C Dictyoglomus thermophilum
2.7.1.90 2.9
-
 D-fructose 1,6-bisphosphate pH 7.2, 50°C Dictyoglomus thermophilum
2.7.1.90 4.3
-
 phosphate pH 7.2, 50°C Dictyoglomus thermophilum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.90 cytosol
-
 Dictyoglomus thermophilum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Ca2+ activates 120% at 0.1 mM Dictyoglomus thermophilum
2.7.1.90 Co2+
-
 Dictyoglomus thermophilum
2.7.1.90 divalent cations activates 120% at 0.1 mM Dictyoglomus thermophilum
2.7.1.90 Fe2+ activates 120% at 0.1 mM Dictyoglomus thermophilum
2.7.1.90 K+ activates 140% at 10 mM Dictyoglomus thermophilum
2.7.1.90 Mg2+ optimal at 0.5-3.5 mM Dictyoglomus thermophilum
2.7.1.90 Na+ activates 135% at 10 mM Dictyoglomus thermophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 37000
-
 2 * 37000, SDS-PAGE Dictyoglomus thermophilum
2.7.1.90 65000
-
 gel filtration Dictyoglomus thermophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Dictyoglomus thermophilum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate Dictyoglomus thermophilum Rt46 B.1
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Dictyoglomus thermophilum
-
-
-
2.7.1.90 Dictyoglomus thermophilum Rt46 B.1
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.1.90 not sensitive to O2 Dictyoglomus thermophilum

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 231fold, to homogeneity Dictyoglomus thermophilum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 24.4
-
 purified enzyme Dictyoglomus thermophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 (polyphosphate)n + D-fructose 6-phosphate n: 12-18 Dictyoglomus thermophilum (polyphosphate)n-1 + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 (polyphosphate)n + D-fructose 6-phosphate 75% of activity with diphosphate Dictyoglomus thermophilum (polyphosphate)n-1 + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 (polyphosphate)n + D-fructose 6-phosphate n: 12-18 Dictyoglomus thermophilum Rt46 B.1 (polyphosphate)n-1 + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Dictyoglomus thermophilum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate equilibrium lies in forward direction Dictyoglomus thermophilum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Dictyoglomus thermophilum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Dictyoglomus thermophilum Rt46 B.1 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate equilibrium lies in forward direction Dictyoglomus thermophilum Rt46 B.1 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Dictyoglomus thermophilum Rt46 B.1 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 triphosphate + D-fructose 6-phosphate 82% of activity with diphosphate Dictyoglomus thermophilum diphosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 triphosphate + D-fructose 6-phosphate 82% of activity with diphosphate Dictyoglomus thermophilum Rt46 B.1 diphosphate + D-fructose 1,6-bisphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.90 dimer 2 * 37000, SDS-PAGE Dictyoglomus thermophilum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.90 PPi-dependent phosphofructokinase
-
 Dictyoglomus thermophilum
2.7.1.90 PPi-PFK
-
 Dictyoglomus thermophilum
2.7.1.90 pyrophosphate-dependent phosphofructokinase
-
 Dictyoglomus thermophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 50
-
 assay at Dictyoglomus thermophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.90 80
-
 half-life: 150 min Dictyoglomus thermophilum
2.7.1.90 90
-
 half-life: 10 min Dictyoglomus thermophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 5.7 6.3 50°C, forward reaction Dictyoglomus thermophilum
2.7.1.90 7 7.5 50°C, reverse reaction Dictyoglomus thermophilum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.90 Dictyoglomus thermophilum
-
-
 4.4