Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • O'Brien, W.E.; Bowien, S.; Wood, H.G.
    Isolation and characterization of a pyrophosphate-dependent phosphofructokinase from Propionibacterium shermanii (1975), J. Biol. Chem., 250, 8690-8695.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 additional information not affected by ATP, AMP, citrate, pyruvate, and malate Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 AMP no inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 ATP no inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 citrate no inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 additional information not affected by ATP, AMP, citrate, pyruvate, and malate Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 phosphoenolpyruvate no inhibition Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.051
-
 D-fructose 1,6-bisphosphate pH 7.4, 25°C Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 0.064
-
 Mn2+ pH 7.4, 25°C Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 0.069
-
 diphosphate pH 7.4, 25°C Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 0.083
-
 Mg2+ pH 7.4, 25°C Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Mg2+ requirement Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 Mn2+ requirement Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 48000
-
 2 * 48000, SDS-PAGE Propionibacterium freudenreichii subsp. shermanii
2.7.1.90 95000
-
 high speed meniscus depletion ultracentrifugation Propionibacterium freudenreichii subsp. shermanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Propionibacterium freudenreichii subsp. shermanii
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Propionibacterium freudenreichii subsp. shermanii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 631fold Propionibacterium freudenreichii subsp. shermanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 245
-
 purified enzyme Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 arsenate + D-fructose 1,6-bisphosphate no substrates are SO42-, MoO42-, SeO42- or ribulose 1,6-bisphosphate Propionibacterium freudenreichii subsp. shermanii ?
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Propionibacterium freudenreichii subsp. shermanii phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Propionibacterium freudenreichii subsp. shermanii phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Propionibacterium freudenreichii subsp. shermanii phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 phosphate + D-glucose 1,6-bisphosphate no activity Propionibacterium freudenreichii subsp. shermanii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.90 dimer 2 * 48000, SDS-PAGE Propionibacterium freudenreichii subsp. shermanii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
 assay at Propionibacterium freudenreichii subsp. shermanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.4
-
 imidazole-HCl buffer, forward and reverse reaction Propionibacterium freudenreichii subsp. shermanii