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Literature summary extracted from

  • Caldecott, K.W.
    Polynucleotide kinase. A versatile molecule makes a clean break (2002), Structure, 10, 1151-1152.
    View publication on PubMed

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA Mammalia involved in repair of chromosomal DNA strand breaks that arise continously, but at low frequency, and are potentially lethal ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA Tequatrovirus T4 involved in repair of broken RNA termini ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 additional information Tequatrovirus T4
-
 ?
-
 ?
2.7.1.78 additional information Mammalia enzyme interacts with XRCC1, a scaffold protein that helps recruit repair enzymes at sites of single-strand breakage ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Mammalia
-
-
-
2.7.1.78 Tequatrovirus T4
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.78 ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA structure of the active sites for 5'-polynucleotide kinase and 3'phosphatase activity of the bifunctional enzyme, substrate binding mechanism Tequatrovirus T4
a
2.7.1.78 ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA reaction mechanism Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA preference for single stranded termini due to the narrow tunnel formation of the active site that can only bind slim molecules Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA preference for recessed termini within duplex DNA Mammalia ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA no preference for overhanging 5'-ends Mammalia ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA involved in repair of chromosomal DNA strand breaks that arise continously, but at low frequency, and are potentially lethal Mammalia ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA involved in repair of broken RNA termini Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 additional information poorly active on recessed termini within duplex DNA Tequatrovirus T4 ?
-
 ?
2.7.1.78 additional information
-
 Tequatrovirus T4 ?
-
 ?
2.7.1.78 additional information enzyme interacts with XRCC1, a scaffold protein that helps recruit repair enzymes at sites of single-strand breakage Mammalia ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 monomer
-
 Mammalia
2.7.1.78 additional information enzyme consists of 2 domains of approximately equal size, separated by a flexible loop into N-terminal half, harboring the 5'-polynucleotide kinase activity, and C-terminal half, harboring the 3'-phosphatase activity, quartenary structure Tequatrovirus T4
2.7.1.78 tetramer
-
 Tequatrovirus T4

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 additional information enzyme also possesses a 3'-phosphatase activity Tequatrovirus T4