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Literature summary extracted from

  • Brunngraber, E.F.
    Nucleoside phosphotransferase from carrot (1978), Methods Enzymol., 51, 387-394.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.77 NaF 0.25 M, enhances activity by about 45% Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.77 Ca2+
-
 Daucus carota
2.7.1.77 cysteine
-
 Daucus carota
2.7.1.77 EDTA
-
 Daucus carota
2.7.1.77 Fe2+
-
 Daucus carota
2.7.1.77 Fe3+
-
 Daucus carota
2.7.1.77 glutathione
-
 Daucus carota
2.7.1.77 Iodine
-
 Daucus carota
2.7.1.77 Mn2+
-
 Daucus carota
2.7.1.77 NaF 0.25 M, phosphatase is inhibited by less than 10% Daucus carota
2.7.1.77 Pb2+
-
 Daucus carota
2.7.1.77 uridine inhibitor of phosphatase reaction Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.77 3.5
-
 Phenylphosphate phosphotransferase reaction with uridine Daucus carota
2.7.1.77 3.5
-
 uridine phosphotransferase reaction with phenyl phosphate Daucus carota

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.77 44000
-
 gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation Daucus carota

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.77 Daucus carota
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.77
-
 Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.77 root
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.77 32
-
-
 Daucus carota

Storage Stability

EC Number Storage Stability Organism
2.7.1.77 -20°C, stable for at least 1 months Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.77 additional information
-
 Daucus carota ?
-
 ?
2.7.1.77 phenyl phosphate + adenosine
-
 Daucus carota AMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + cytidine
-
 Daucus carota CMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + deoxyadenosine
-
 Daucus carota dAMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + deoxycytidine
-
 Daucus carota dCMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + deoxyguanosine
-
 Daucus carota dGMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + deoxyuridine
-
 Daucus carota dUMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + guanosine
-
 Daucus carota GMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + thymidine
-
 Daucus carota TMP + phenol
-
 ?
2.7.1.77 phenyl phosphate + uridine
-
 Daucus carota 5'-UMP + phenol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.77 5
-
-
 Daucus carota

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.77 9.5
-
 without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h Daucus carota

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.77 Daucus carota intact enzyme
-
 4.1
2.7.1.77 Daucus carota subunit A 4.7 4.6
2.7.1.77 Daucus carota subunit B
-
 5.1