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Literature summary extracted from

  • Deibler, R.D.; Reznik, R.B.; Ives, D.H.
    Deoxynucleoside kinases from Lactobacilli. Separate interacting sites for deoxycytidine and deoxyadenosine (1977), J. Biol. Chem., 252, 8240-8244.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.76 2'-O-Cyclocytidine activation Lactobacillus acidophilus
2.7.1.76 5-Fluoro-2'-deoxycytidine 0.4 mM, 275% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.028 mM Lactobacillus acidophilus
2.7.1.76 cytosine arabinoside activation Lactobacillus acidophilus
2.7.1.76 deoxycytidine 30 mM, 584% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.1 mM Lactobacillus acidophilus
2.7.1.76 deoxycytidine monophosphate 10 mM, 409% activation of deoxyadenosine phosphorylation, half-maximal activation at 1.6 mM Lactobacillus acidophilus
2.7.1.76 deoxyguanosine 5 mM, 330% activation of deoxyadenosine phosphorylation, half-maximal activation at 2.2 mM Lactobacillus acidophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.74 5-Fluoro-2'-deoxycytidine
-
 Lactobacillus acidophilus
2.7.1.74 cytidine
-
 Lactobacillus acidophilus
2.7.1.74 dATP not Lactobacillus acidophilus
2.7.1.74 dCTP ATP as substrate; moderate phosphate donor in the absence of ATP Lactobacillus acidophilus
2.7.1.74 deoxyadenosine cytosolic isozyme I, weak; deoxycytidine; kinetics Lactobacillus acidophilus
2.7.1.74 deoxyguanosine
-
 Lactobacillus acidophilus
2.7.1.74 dGTP ATP as substrate Lactobacillus acidophilus
2.7.1.74 dTTP ATP as substrate; weak Lactobacillus acidophilus
2.7.1.74 Trypsin substrates or dNTP protect corresponding active site Lactobacillus acidophilus
2.7.1.76 adenine arabinoside
-
 Lactobacillus acidophilus
2.7.1.76 adenosine
-
 Lactobacillus acidophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.74 additional information
-
 additional information kinetic study Lactobacillus acidophilus
2.7.1.76 additional information
-
 additional information kinetic study Lactobacillus acidophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.74 Mg2+
-
 Lactobacillus acidophilus
2.7.1.76 Mg2+ required for activity Lactobacillus acidophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.74 Lactobacillus acidophilus
-
-
-
2.7.1.76 Lactobacillus acidophilus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.74 NTP + deoxycytidine = NDP + dCMP mechanism Lactobacillus acidophilus
a
2.7.1.76 ATP + 2'-deoxyadenosine = ADP + dAMP random sequential substrate addition Lactobacillus acidophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.74 ATP + 2'-deoxycytidine
-
 Lactobacillus acidophilus ADP + 5'-dCMP
-
 ?
2.7.1.74 dCTP + 2'-deoxycytidine only in the absence of ATP Lactobacillus acidophilus dCDP + 2'-deoxy-CMP
-
 ?
2.7.1.74 additional information enzyme has two separate active sites for deoxycytidine and deoxyadenosine activity Lactobacillus acidophilus ?
-
 ?
2.7.1.74 NTP + deoxyadenosine
-
 Lactobacillus acidophilus NDP + dAMP
-
 ?
2.7.1.74 NTP + deoxycytidine
-
 Lactobacillus acidophilus NDP + dCMP
-
 ?
2.7.1.76 ATP + deoxyadenosine enzyme bears two separate but interacting active sites for deoxyadenosine and deoxycytidine kinase activity Lactobacillus acidophilus ADP + dAMP
-
 ?
2.7.1.76 ATP + deoxycytidine
-
 Lactobacillus acidophilus ADP + dCMP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.76 20
-
 assay at Lactobacillus acidophilus