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Literature summary extracted from

  • Kai, M.; Salway, J.G.; Hawthorne, J.N.
    The diphosphoinositide kinase of rat brain (1968), Biochem. J., 106, 791-801.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.68 Acetylcholine 0.01 mM or 0.1 mM, each in presence of 0.1 mM eserine, stimulates the enzyme in homogenates by 30-40% Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.68 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate product inhibition Rattus norvegicus
2.7.1.68 Ca2+ inhibits in presence of optimal Mg2+ concentrations Rattus norvegicus
2.7.1.68 cysteine 1 mM, 49% inhibition Rattus norvegicus
2.7.1.68 GSSG 0.1 mM, 17% inhibition Rattus norvegicus
2.7.1.68 NEM 1 mM, 26% inhibition Rattus norvegicus
2.7.1.68 PCMB 0.1 mM, complete inhibition Rattus norvegicus
2.7.1.68 Triton X-100 0.5-3.0% w/v, 40% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.68 0.25
-
 1-phosphatidyl-1D-myo-inositol 4-phosphate pH 7.4, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.68 Ca2+ activates to a smaller extent than Mg2+ Rattus norvegicus
2.7.1.68 Co2+ activates to a smaller extent than Mg2+ Rattus norvegicus
2.7.1.68 Mg2+ activates Rattus norvegicus
2.7.1.68 Mn2+ activates to a smaller extent than Mg2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.68 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.68
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.68 brain
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.68 ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Rattus norvegicus ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.68 7.3
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.68 6.1 7.9 pH 6.1: about 85% of maximal activity, pH 7.9: about 85% of maximal activity Rattus norvegicus