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Literature summary extracted from

  • Sandermann, H.; Strominger, J.L.
    Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus (1972), J. Biol. Chem., 247, 5123-5131.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.66 Phospholipid cofactor
-
 Staphylococcus aureus

General Stability

EC Number General Stability Organism
2.7.1.66 enzyme is quite stable to SDS, recovered from SDS gel slices after solubilization in 1% SDS, 1% 2-mercaptoethanol Staphylococcus aureus
2.7.1.66 remarkably stable towards SDS, 8 M urea and SH-reagents Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.66 1-butanol
-
 Staphylococcus aureus
2.7.1.66 guanidine hydrochloride strong inhibition above 100 mM Staphylococcus aureus
2.7.1.66 NaCl above 0.5 M Staphylococcus aureus
2.7.1.66 Urea 8 M, 20% inhibition Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.66 0.057
-
 ATP pH 8.5, 25°C, reaction with C55-isoprenoid alcohols from Streptococcus faecalis or ficaprenol Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.66 membrane
-
 Staphylococcus aureus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.66 NaCl 0.5 M, stimulates about 2-fold Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.66 17000
-
 apoprotein, gel filtration Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.66 Staphylococcus aureus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.66 lipoprotein
-
 Staphylococcus aureus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.66
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.66 0.54
-
-
 Staphylococcus aureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.66 100
-
 30 min, about 30% loss of activity Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.66 8.5
-
-
 Staphylococcus aureus