BRENDA - Enzyme Database

Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus

Sandermann, H.; Strominger, J.L.; J. Biol. Chem. 247, 5123-5131 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.66
Phospholipid cofactor
-
Staphylococcus aureus
General Stability
EC Number
General Stability
Organism
2.7.1.66
enzyme is quite stable to SDS, recovered from SDS gel slices after solubilization in 1% SDS, 1% 2-mercaptoethanol
Staphylococcus aureus
2.7.1.66
remarkably stable towards SDS, 8 M urea and SH-reagents
Staphylococcus aureus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.66
1-butanol
-
Staphylococcus aureus
2.7.1.66
guanidine hydrochloride
strong inhibition above 100 mM
Staphylococcus aureus
2.7.1.66
NaCl
above 0.5 M
Staphylococcus aureus
2.7.1.66
Urea
8 M, 20% inhibition
Staphylococcus aureus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.66
0.057
-
ATP
pH 8.5, 25░C, reaction with C55-isoprenoid alcohols from Streptococcus faecalis or ficaprenol
Staphylococcus aureus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.66
membrane
-
Staphylococcus aureus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.66
NaCl
0.5 M, stimulates about 2-fold
Staphylococcus aureus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.66
17000
-
apoprotein, gel filtration
Staphylococcus aureus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
2.7.1.66
Staphylococcus aureus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.66
lipoprotein
-
Staphylococcus aureus
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
2.7.1.66
-
Staphylococcus aureus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.66
0.54
-
-
Staphylococcus aureus
Temperature Stability [░C]
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
2.7.1.66
100
-
30 min, about 30% loss of activity
Staphylococcus aureus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.66
8.5
-
-
Staphylococcus aureus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.66
Phospholipid cofactor
-
Staphylococcus aureus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.66
enzyme is quite stable to SDS, recovered from SDS gel slices after solubilization in 1% SDS, 1% 2-mercaptoethanol
Staphylococcus aureus
2.7.1.66
remarkably stable towards SDS, 8 M urea and SH-reagents
Staphylococcus aureus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.66
1-butanol
-
Staphylococcus aureus
2.7.1.66
guanidine hydrochloride
strong inhibition above 100 mM
Staphylococcus aureus
2.7.1.66
NaCl
above 0.5 M
Staphylococcus aureus
2.7.1.66
Urea
8 M, 20% inhibition
Staphylococcus aureus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.66
0.057
-
ATP
pH 8.5, 25░C, reaction with C55-isoprenoid alcohols from Streptococcus faecalis or ficaprenol
Staphylococcus aureus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.66
membrane
-
Staphylococcus aureus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.66
NaCl
0.5 M, stimulates about 2-fold
Staphylococcus aureus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.66
17000
-
apoprotein, gel filtration
Staphylococcus aureus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.66
lipoprotein
-
Staphylococcus aureus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.66
-
Staphylococcus aureus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.1.66
0.54
-
-
Staphylococcus aureus
Temperature Stability [░C] (protein specific)
EC Number
Temperature Stability Minimum [░C]
Temperature Stability Maximum [░C]
Commentary
Organism
2.7.1.66
100
-
30 min, about 30% loss of activity
Staphylococcus aureus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.66
8.5
-
-
Staphylococcus aureus