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Literature summary extracted from

  • Sandermann, H.; Strominger, J.L.
    C55-isoprenoid alcohol phosphokinase: an enzyme soluble in organic solvents (1974), Methods Enzymol., 32, 439-446.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.66 Phospholipid cofactor phospholipid cofactor required Staphylococcus aureus

General Stability

EC Number General Stability Organism
2.7.1.66 remarkably stable towards SDS, 8 M urea and SH-reagents Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.66 guanidine hydrochloride
-
 Staphylococcus aureus
2.7.1.66 NaCl above 0.5 M Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.66 0.057
-
 ATP
-
 Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.66 membrane
-
 Staphylococcus aureus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.66 NaCl 0.5 M, stimulates about 2-fold Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.66 17000
-
 apoprotein, gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.66 additional information Staphylococcus aureus the product of the reaction, C55-isoprenoid alcohol monophosphate functions in the biosynthesis of bacterial peptidoglycan and lipopolysaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.66 Staphylococcus aureus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.1.66 lipoprotein phospholipid cofactor required Staphylococcus aureus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.66
-
 Staphylococcus aureus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.66 additional information
-
-
 Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.66 ATP + C55-isoprenoid alcohol
-
 Staphylococcus aureus ADP + C55-isoprenoid alcohol monophosphate
-
 ?
2.7.1.66 ATP + ficaprenol
-
 Staphylococcus aureus ADP + ficaprenyl phosphate
-
 ?
2.7.1.66 ATP + solanesol poor substrate Staphylococcus aureus ADP + solanesyl phosphate
-
 ?
2.7.1.66 additional information the product of the reaction, C55-isoprenoid alcohol monophosphate functions in the biosynthesis of bacterial peptidoglycan and lipopolysaccharides Staphylococcus aureus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.66 monomer 1 * 17000, SDS-PAGE Staphylococcus aureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.66 100
-
 30 min, about 30% loss of activity Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.66 8.5
-
-
 Staphylococcus aureus