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Literature summary extracted from

  • Higashi, Y.; Siewert, G.; Strominger, J.L.
    Biosynthesis of the peptidoglycan of bacterial cell walls. XIX. Isoprenoid alcohol phosphokinase (1970), J. Biol. Chem., 245, 3683-3690.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.66 relatively unstable in suspension in water Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.66 Mn2+ complete inhibition at 0.01 M Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.66 0.4
-
 ATP pH 8.2, 37°C, reaction with ficaprenol Staphylococcus aureus
2.7.1.66 0.5
-
 ficaprenol pH 8.2, 37°C Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.66 membrane exclusively found in the cell membrane fraction Staphylococcus aureus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.66 Co2+ no effect Staphylococcus aureus
2.7.1.66 Mg2+ optimum concentration: 0.01 M Staphylococcus aureus
2.7.1.66 Mg2+ divalent cation required, Mg2+ most effective, optimal concentration: 0.01 M Staphylococcus aureus
2.7.1.66 Mn2+ can partially replace Mg2+ in activation Staphylococcus aureus
2.7.1.66 Mn2+ one-fourth of the activity of Mg2+ at 0.001 M Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.66 additional information Staphylococcus aureus product of the reaction is a substrate for the synthesis of lipid intermediates in peptidoglycan synthesis ?
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.1.66 1-butanol highly stable Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.66 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.66 ATP + ficaprenol
-
 Staphylococcus aureus ADP + ficaprenyl phosphate
-
 ?
2.7.1.66 ATP + solanesol no activity Staphylococcus aureus ADP + solanesyl phosphate
-
 ?
2.7.1.66 additional information product of the reaction is a substrate for the synthesis of lipid intermediates in peptidoglycan synthesis Staphylococcus aureus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.66 100
-
 pH 8.2, aqueous suspension in Tris-HCl, presence of substrate, t1/2: 10 min Staphylococcus aureus
2.7.1.66 100
-
 solution in 1-butanol, activation of nearly 50% after 20 min Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.66 8.2
-
-
 Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.66 7.2 8.8 pH 7.2: about 80% of maximal activity, pH 8.8: about 70% of maximal activity Staphylococcus aureus