EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.7 | Polyphosphate | dependent on, can be substituted by ATP, nonprocessive utilization, binding site is the same as for ATP binding | Arthrobacter sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.7 | DNA and amino acid sequence determination and analysis, phylogenetic analysis | Arthrobacter sp. |
2.7.1.63 | - |
Arthrobacter sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.7 | 0.02 | - |
Hexametaphosphate | pH 7.0 | Arthrobacter sp. | |
2.7.1.7 | 0.2 | - |
ATP | pH 7.0 | Arthrobacter sp. | |
2.7.1.7 | 0.5 | - |
D-glucose | pH 7.0 | Arthrobacter sp. | |
2.7.1.7 | 15 | - |
D-mannose | pH 7.0 | Arthrobacter sp. | |
2.7.1.63 | 0.02 | - |
Hexametaphosphate | pH 7.0, 30°C | Arthrobacter sp. | |
2.7.1.63 | 0.15 | - |
mannose | pH 7.0, 30°C | Arthrobacter sp. | |
2.7.1.63 | 0.2 | - |
ATP | pH 7.0, 30°C | Arthrobacter sp. | |
2.7.1.63 | 0.5 | - |
glucose | pH 7.0, 30°C | Arthrobacter sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.7 | Co2+ | activates, 86% and 30% activity with polyphosphate and ATP, respectively, compared to Mg2+ | Arthrobacter sp. | |
2.7.1.7 | Cu2+ | activates, 11% activity with polyphosphate compared to Mg2+, no activity with ATP | Arthrobacter sp. | |
2.7.1.7 | Fe2+ | activates, 15% activity with ATP compared to Mg2+, no activity with polyphosphate | Arthrobacter sp. | |
2.7.1.7 | Mg2+ | absolutely required, most effective metal ion | Arthrobacter sp. | |
2.7.1.7 | Mn2+ | activates, 82% and 88% activity with polyphosphate and ATP, respectively, compared to Mg2+ | Arthrobacter sp. | |
2.7.1.7 | additional information | activities with different divalent cations in descending order: Mg2+, Mn2+, Co2+, Zn2+, Cu2+, and Fe2+, no activity with Hg2+ | Arthrobacter sp. | |
2.7.1.7 | Zn2+ | activates, 80% and 19% activity with polyphosphate and ATP, respectively, compared to Mg2+ | Arthrobacter sp. | |
2.7.1.63 | Co2+ | divalent cation required, 86% of the activity with Mg2+ for the polyphosphate-dependent activity and 30% of the activity with Mg2+ for ATP-dependent activity, 5 mM | Arthrobacter sp. | |
2.7.1.63 | Cu2+ | divalent cation required, 11% of the activity with Mg2+ for the polyphosphate-dependent activity and no activation of ATP-dependent activity, 5 mM | Arthrobacter sp. | |
2.7.1.63 | Fe2+ | divalent cation required, no activation of the polyphosphate-dependent activity and 15% of the activity with Mg2+ for ATP-dependent acticity, 5 mM | Arthrobacter sp. | |
2.7.1.63 | Mg2+ | divalent cation required, maximal activition of polyphosphate-dependent glucokinase and ATP-dependent glucokinase activity, 5 mM | Arthrobacter sp. | |
2.7.1.63 | Mn2+ | divalent cation required, 82% of the activity with Mg2+ for the polyphosphate-dependent activity and 88% of the activity with Mg2+ for ATP-dependent activity, 5 mM | Arthrobacter sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 30000 | - |
- |
Arthrobacter sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.7 | Arthrobacter sp. | Q7WT42 | - |
- |
2.7.1.7 | Arthrobacter sp. KM | Q7WT42 | - |
- |
2.7.1.63 | Arthrobacter sp. | - |
- |
- |
2.7.1.63 | Arthrobacter sp. KM | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.7 | native enzyme 2200fold and 1375fold determined with polyphosphate and ATP, respectively, to homogeneity | Arthrobacter sp. |
2.7.1.63 | - |
Arthrobacter sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.7 | ATP + D-mannose = ADP + D-mannose 6-phosphate | mechanism, enzyme shows both mannokinase and glucokinase activities | Arthrobacter sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 0.064 | - |
mannokinase activity with polyphosphate in cell extract | Arthrobacter sp. |
2.7.1.7 | 0.077 | - |
mannokinase activity with ATP in cell extract | Arthrobacter sp. |
2.7.1.7 | 110 | - |
purified enzyme, mannokinase activity with ATP | Arthrobacter sp. |
2.7.1.7 | 220 | - |
purified enzyme, mannokinase activity with polyphosphate | Arthrobacter sp. |
2.7.1.63 | 110 | - |
reaction with ATP and glucose | Arthrobacter sp. |
2.7.1.63 | 220 | - |
reaction with polyphosphate and glucose | Arthrobacter sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.7 | ATP + D-glucose | - |
Arthrobacter sp. | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-glucose | - |
Arthrobacter sp. KM | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-mannose | - |
Arthrobacter sp. | ADP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | ATP + D-mannose | - |
Arthrobacter sp. KM | ADP + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | hexametaphosphate + D-mannose | - |
Arthrobacter sp. | pentaphosphate + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | hexametaphosphate + D-mannose | - |
Arthrobacter sp. KM | pentaphosphate + D-mannose 6-phosphate | - |
? | |
2.7.1.7 | additional information | enzyme is also active with D-glucose, which is a more efficient substrate than D-mannose, ratio 3:2 and 3:1 with ATP and polyphosphate, respectively | Arthrobacter sp. | ? | - |
? | |
2.7.1.7 | additional information | enzyme is also active with D-glucose, which is a more efficient substrate than D-mannose, ratio 3:2 and 3:1 with ATP and polyphosphate, respectively | Arthrobacter sp. KM | ? | - |
? | |
2.7.1.63 | (phosphate)n + D-glucose | - |
Arthrobacter sp. | (phosphate)n-1 + D-glucose 6-phosphate | - |
ir | |
2.7.1.63 | (phosphate)n + D-glucose | - |
Arthrobacter sp. KM | (phosphate)n-1 + D-glucose 6-phosphate | - |
ir | |
2.7.1.63 | (phosphate)n + D-mannose | about 40% of the activity with glucose | Arthrobacter sp. | (phosphate)n-1 + D-mannose 6-phosphate | - |
? | |
2.7.1.63 | (phosphate)n + D-mannose | about 40% of the activity with glucose | Arthrobacter sp. KM | (phosphate)n-1 + D-mannose 6-phosphate | - |
? | |
2.7.1.63 | ATP + D-glucose | - |
Arthrobacter sp. | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | ATP + D-glucose | - |
Arthrobacter sp. KM | ADP + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | ATP + D-mannose | about 30% of the activity with glucose | Arthrobacter sp. | ADP + D-mannose 6-phosphate | - |
? | |
2.7.1.63 | ATP + D-mannose | about 30% of the activity with glucose | Arthrobacter sp. KM | ADP + D-mannose 6-phosphate | - |
? | |
2.7.1.63 | hexametaphosphate + D-glucose | - |
Arthrobacter sp. | pentametaphosphate + D-glucose 6-phosphate | - |
? | |
2.7.1.63 | additional information | the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP | Arthrobacter sp. | ? | - |
? | |
2.7.1.63 | additional information | the enzyme also shows weak NAD kinase activity and fructokinase activity with polyphosphate or ATP | Arthrobacter sp. KM | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.7 | monomer | 1 * 30000 | Arthrobacter sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.7 | inorganic polyphosphate/ATP-glucomannokinase | - |
Arthrobacter sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 45 | - |
- |
Arthrobacter sp. |
2.7.1.63 | 45 | - |
polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity | Arthrobacter sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 40 | - |
loss of 50% activity after 5 min | Arthrobacter sp. |
2.7.1.63 | 40 | - |
5 min, 50% loss of activity | Arthrobacter sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.7 | 7.5 | - |
- |
Arthrobacter sp. |
2.7.1.63 | 7.5 | - |
polyphosphate- and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity | Arthrobacter sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.63 | 6 | 9.5 | pH 6.0: about 25% of maximal activity, pH 9.5: about 35% of maximal activity, polyphosphate-dependent and ATP-dependent mannokinase activity, polyphosphate and ATP-dependent glucokinase activity | Arthrobacter sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.7 | ATP | dependent on, can be substituted by polyphosphate, binding site is the same as for polyphosphate binding | Arthrobacter sp. |