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Literature summary extracted from

  • Hsieh, P.C.; Kowalczyk, T.H.; Phillips, N.F.
    Kinetic mechanisms of polyphosphate glucokinase from Mycobacterium tuberculosis (1996), Biochemistry, 35, 9772-9781.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.63 ADP inhibits reaction with ATP Mycobacterium tuberculosis
2.7.1.63 AMP inhibits reaction with polyphosphate and glucose or ATP and glucose Mycobacterium tuberculosis
2.7.1.63 ATP at high concentrations competitive substrate inhibition with respect to glucose, ATP-dependent reaction Mycobacterium tuberculosis
2.7.1.63 D-fructose 6-phosphate inhibits reaction with polyphosphate and glucose or ATP and glucose Mycobacterium tuberculosis
2.7.1.63 D-glucose 6-phosphate inhibits reaction with polyphosphate and glucose Mycobacterium tuberculosis
2.7.1.63 D-xylose inhibits reaction with ATP; inhibits reaction with polyphosphate and glucose or ATP and glucose Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.63 0.06
-
 D-glucose pH 7.5, reaction with ATP Mycobacterium tuberculosis
2.7.1.63 0.22
-
 D-glucose pH 8.6, reaction with ATP Mycobacterium tuberculosis
2.7.1.63 0.88
-
 ATP pH 7.5 Mycobacterium tuberculosis
2.7.1.63 1.4
-
 ATP pH 8.6 Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Mycobacterium tuberculosis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.63 (phosphate)n + D-glucose = (phosphate)n-1 + D-glucose 6-phosphate in the polyphosphate-dependent reaction the enzyme follows an ordered bi bi sequential mechanism with polyphosphate binding to the enzyme first and glucose 6-phosphate dissociating last. The ATP-dependent glucokinase reaction is consistent with an ordered bi bi sequential mechanism, with ATP binding to the enzyme first and glucose 6-phosphate leaving last Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)n + D-glucose polyphosphate is utilized nonprocessively with a preference for longer chains Mycobacterium tuberculosis (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 ATP + D-glucose
-
 Mycobacterium tuberculosis ADP + D-glucose 6-phosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.63 108
-
 ATP pH 7.5 Mycobacterium tuberculosis
2.7.1.63 108
-
 glucose pH 7.5 Mycobacterium tuberculosis
2.7.1.63 116
-
 ATP pH 8.6 Mycobacterium tuberculosis
2.7.1.63 116
-
 glucose pH 8.6 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.63 6.6
-
 ATP pH 7.5 Mycobacterium tuberculosis
2.7.1.63 7.4
-
 ATP pH 8.6 Mycobacterium tuberculosis