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Literature summary extracted from

  • Girbal, E.; Binot, R.A.; Monsan, P.F.
    Production, purification, properties and kinetic studies of free and immobilized polyphosphate:glucose-6-phosphotransferase from Mycobacterium phlei (1989), Enzyme Microb. Technol., 11, 518-527.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.63 dialysed enzyme preparations are largely inactive, Mg2+ restores activity Mycolicibacterium phlei
2.7.1.63 enzyme immobilized on corn stover is stable for one months at pH 8.5, 30°C Mycolicibacterium phlei

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.63 D-glucose above 150 mM, substrate inhibition Mycolicibacterium phlei
2.7.1.63 Mg2+ molar ratio polyphosphate/Mg below 0.5, activation above, EDTA partially reverses Mycolicibacterium phlei
2.7.1.63 Poly(P) above 0.05 mM, substrate inhibition Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.63 0.0072
-
 (phosphate)15 pH 8.5, 30°C, soluble enzyme Mycolicibacterium phlei
2.7.1.63 0.13
-
 (phosphate)15 pH 8.5, 30°C, immobilized enzyme Mycolicibacterium phlei
2.7.1.63 0.48
-
 D-glucose pH 8.5, 30°C, soluble enzyme Mycolicibacterium phlei
2.7.1.63 0.6
-
 D-glucose pH 8.5, 30°C, immobilized enzyme Mycolicibacterium phlei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.63 cytosol
-
 Mycolicibacterium phlei 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.63 Ca2+ activation, about 30% as efficient as Mg2+ Mycolicibacterium phlei
2.7.1.63 Mg2+ activation Mycolicibacterium phlei
2.7.1.63 Mg2+ molar ratio poly(P)/Mg2+ above 0.5 inhibition below Mycolicibacterium phlei
2.7.1.63 Mn2+ activation, as effective as Mg2+ Mycolicibacterium phlei
2.7.1.63 Zn2+ activation, about 30% as efficient as Mg2+ Mycolicibacterium phlei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.63 34000
-
 x * 34000, SDS-PAGE Mycolicibacterium phlei
2.7.1.63 275000 280000 non-denaturing PAGE, gel filtration Mycolicibacterium phlei

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.63 Mycobacterium gordonae
-
-
-
2.7.1.63 Mycolicibacterium aurum
-
-
-
2.7.1.63 Mycolicibacterium phlei
-
-
-
2.7.1.63 Mycolicibacterium smegmatis
-
-
-
2.7.1.63 Mycolicibacterium thermoresistibile
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.63
-
 Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.63 140
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.63 (phosphate)15 + D-glucose
-
 Mycolicibacterium phlei (phosphate)14 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Mycolicibacterium smegmatis (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Mycolicibacterium aurum (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Mycobacterium gordonae (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose
-
 Mycolicibacterium thermoresistibile (phosphate)n-1 + D-glucose 6-phosphate
-
 ?
2.7.1.63 (phosphate)n + D-glucose i.e. poly(P)n, no substrates are monophosphate, diphosphate or triphosphate Mycolicibacterium phlei (phosphate)n-1 + D-glucose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.63 ? x * 34000, SDS-PAGE Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.63 6.4 8.3 broad plateau Mycolicibacterium phlei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.63 6 8.4 very little activity below pH 6.0 and above pH 8.4 Mycolicibacterium phlei

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.63 8.1 8.4 immobilized enzyme is stable Mycolicibacterium phlei

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.63 Mycolicibacterium phlei isoelectric focusing
-
 5