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Literature summary extracted from

  • Banerjee, S.; Ghosh, S.
    N-Acetylmannosamnine kinase (1975), Methods Enzymol., 42c, 53-58.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.60 fairly stable, also stable to dialysis Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.60 0.75
-
 N-acetyl-D-mannosamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.60 1.15
-
 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.60 Co2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.60 Mg2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.60 Mn2+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.1.60 additional information Ca2+, Na+ and K+ have no effect Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.60 ATP + N-acetyl-D-mannosamine Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.60 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.60
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.60 19.3
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.7.1.60 4°C, loses about 30% of its activity during 7 days of storage Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.60 ATP + N-acetyl-D-mannosamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + N-acetyl-D-mannosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acetyl-D-mannosamine
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + N-acetyl-D-mannosamine-6-phosphate
-
 ?
2.7.1.60 additional information no enzyme found when bacteria grow in presence of glucose Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.60 6.2 8
-
 Salmonella enterica subsp. enterica serovar Typhimurium