Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Veiga-da-Cunha, M.; Houyoux, A.; Van Schaftingen, E.
    Overexpression and purification of fructose-1-phosphate kinase from Escherichia coli: Application to the assay of fructose 1-phosphate (2000), Protein Expr. Purif., 19, 48-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.56 overexpression in Escherichia coli Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.56 0.13
-
D-Fructose 1-phosphate pH 7.1, 30°C, in the presence of 50 mM KCl Escherichia coli
2.7.1.56 0.35
-
ATP pH 7.1, 30°C, in the presence of 5 mM KCl Escherichia coli
2.7.1.56 0.36
-
fructose 1-phosphate pH 7.1, 30°C, in the absence of KCl Escherichia coli
2.7.1.56 0.6
-
ATP pH 7.1, 30°C, in the presence of 50 mM KCl Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 K+ 50 mM, 3fold increase of maximal activity Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.56 34000
-
2 * 34000, recombinant enzyme, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.56 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.56 ammonium sulfate, Sephadex G25, DEAE-Sepharose Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.56 -80°C, 20% glycerol, 2 years, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate
-
Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.56 dimer 2 * 34000, recombinant enzyme, SDS-PAGE Escherichia coli