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Literature summary extracted from

  • Buschmeier, B.; Hengstenberg, W.; Deutscher, J.
    Purification and properties of 1-phosphofructokinase from Escherichia coli (1985), FEMS Microbiol. Lett., 29, 231-235.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.56 ADP kinetics Escherichia coli
2.7.1.56 D-fructose 1,6-bisphosphate kinetics Escherichia coli
2.7.1.56 additional information not inhibited by D-fructose 6-phosphate; not inhibited by D-glucose 1-phosphate; not inhibited by D-glucose 6-phosphate Escherichia coli
2.7.1.56 phenylmethanesulfonyl fluoride 1 mM, 65% irreversible inhibition, 1.5 M ATP protect Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.56 0.12
-
 ATP pH 7.6, 37°C Escherichia coli
2.7.1.56 0.25
-
 D-Fructose 1-phosphate pH 7.6, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 Co2+ 5 mM, 25% of activity with Mg2+ Escherichia coli
2.7.1.56 Mg2+ required for activity Escherichia coli
2.7.1.56 Mn2+ required for activity Escherichia coli
2.7.1.56 Mn2+ 5 mM, 50% of activity with Mg2+ Escherichia coli
2.7.1.56 additional information not activated by Ca2+, Cu2+ and Fe2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.56 30000
-
 2 * 30000, SDS-PAGE Escherichia coli
2.7.1.56 57000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.56 Escherichia coli
-
 K12 strain jOD5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.56 DEAE-cellulose, ammonium sulfate, hydroxylapatite, Sephadex G-75, DEAE-cellulose Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ATP + D-fructose 1-phosphate no activity with D-glucose 6-phosphate, D-glucose 1-phosphate Escherichia coli ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 CTP + D-fructose 1-phosphate 10% of activity with ATP Escherichia coli CDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 GTP + D-fructose 1-phosphate 60% of activity with ATP Escherichia coli GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 UTP + D-fructose 1-phosphate 20% of activity with ATP Escherichia coli UDP + D-fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.56 dimer 2 * 30000, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.56 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.56 7.8
-
 50 mM Tris-HCl Escherichia coli
2.7.1.56 8.5
-
 50 mM NH4HCO3 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.56 0.28
-
 ADP pH 7.6, 37°C Escherichia coli
2.7.1.56 6.1
-
 D-fructose-1,6-bisphosphate pH 7.6, 37°C Escherichia coli