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Literature summary extracted from

  • Reeves, R.E.; Warren, L.G.; Hsu, D.S.
    1-Phosphofructokinase from an anaerobe (1966), J. Biol. Chem., 241, 1257-1261.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.56 dialysis against water, 0.9% NaCl or buffer, stable to [Clostridium] symbiosum
2.7.1.56 partially purified enzyme withstands freeze-thawing, purified enzyme loses 50% of activity by freezing and thawing [Clostridium] symbiosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.56 additional information not inhibited by D-fructose 6-phosphate; not inhibited by sorbose 1-phosphate [Clostridium] symbiosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.56 soluble
-
 [Clostridium] symbiosum
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.56 Mg2+ required for activity [Clostridium] symbiosum
2.7.1.56 Mn2+ can replace Mg2+ [Clostridium] symbiosum
2.7.1.56 Mn2+ required for activity [Clostridium] symbiosum
2.7.1.56 additional information not activated by Ca2+ [Clostridium] symbiosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate [Clostridium] symbiosum inducible enzyme ADP + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.56 [Clostridium] symbiosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.56 partial [Clostridium] symbiosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.56 6.9 81.6
-
 [Clostridium] symbiosum

Storage Stability

EC Number Storage Stability Organism
2.7.1.56 4°C, a few days [Clostridium] symbiosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.56 ATP + D-fructose 1-phosphate no activity with D-fructose 6-phosphate [Clostridium] symbiosum ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ATP + D-fructose 1-phosphate no activity with sorbose 1-phosphate [Clostridium] symbiosum ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ATP + D-fructose 1-phosphate inducible enzyme [Clostridium] symbiosum ADP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 GTP + D-fructose 1-phosphate
-
 [Clostridium] symbiosum GDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 ITP + D-fructose 1-phosphate
-
 [Clostridium] symbiosum IDP + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.56 UTP + D-fructose 1-phosphate
-
 [Clostridium] symbiosum UDP + D-fructose 1,6-bisphosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.56 25
-
 assay at [Clostridium] symbiosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.56 8 8.8 50% activity at pH 7.1 [Clostridium] symbiosum