Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kilker, R.D.; Shuey, D.K.; Serif, G.S.
    Isolation and properties of porcine thyroid fucokinase (1979), Biochim. Biophys. Acta, 570, 271-283.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.52 GDP-alpha-D-mannose stimulation Sus scrofa

General Stability

EC Number General Stability Organism
2.7.1.52 unstable in the presence of KCl Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.52 ADP 4.9 mM, 96% inhibition, competitive with ATP Sus scrofa
2.7.1.52 AMP 5.0 mM, 14% inhibition in presence of ATP Sus scrofa
2.7.1.52 CDP 4.9 mM, 16% inhibition in presence of ATP Sus scrofa
2.7.1.52 CTP 4.9 mM, 30% inhibition in presence of ATP Sus scrofa
2.7.1.52 GDP-L-fucose competitive with L-fucose Sus scrofa
2.7.1.52 GTP 0.1 mM, 59% inhibition in presence of ATP Sus scrofa
2.7.1.52 TTP 4.8 mM, 45% inhibition in presence of ATP Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 0.03
-
 L-fucose pH 7.4, 37°C Sus scrofa
2.7.1.52 1
-
 ATP pH 7.4, 37°C Sus scrofa
2.7.1.52 1
-
 ATP ATP in form of MgATP2- Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.52 78000
-
 4 * 78000, SDS-PAGE Sus scrofa
2.7.1.52 309200
-
 gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose Sus scrofa i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.52
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.52 thyroid gland
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.52 0.6
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.52 -20°C, in the presence of 2-mercaptoethanol and glycerol, quite stable Sus scrofa
2.7.1.52 4°C, unstable in saline solution Sus scrofa
2.7.1.52 frozen, unstable in saline solution Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose highly specific for ATP Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?
2.7.1.52 ATP + 6-deoxy-L-galactose highly specific for L-fucose Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?
2.7.1.52 ATP + 6-deoxy-L-galactose i.e. L-fucose, serves to utilize free L-fucose for glycoprotein synthesis Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate
-
 ?
2.7.1.52 ATP + L-fucose
-
 Sus scrofa ADP + beta-L-fucose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.52 tetramer 4 * 78000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.52 6.5
-
-
 Sus scrofa
2.7.1.52 6.6
-
-
 Sus scrofa