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Literature summary extracted from

  • Ishihara, H.; Massaro, D.J.; Heath, E.C.
    The metabolism of L-fucose. 3. The enzymatic synthesis of beta-L-fucose 1-phosphate (1968), J. Biol. Chem., 243, 1103-1109.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.52 ovalbumin stabilizes Sus scrofa
2.7.1.52 substrates do not stabilize Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.52 0.12
-
L-fucose pH 8, 37°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.52 Ca2+ absolute requirement for a divalent cation, 85% of the activation with Mg2+ Sus scrofa
2.7.1.52 Co2+ absolute requirement for a divalent cation, 15% of the activation with Mg2+ Sus scrofa
2.7.1.52 Fe2+ absolute requirement for a divalent cation, 29% of the activation with Mg2+ Sus scrofa
2.7.1.52 Mg2+ absolute requirement for a divalent cation, Mg2+ is most efficient Sus scrofa
2.7.1.52 Mn2+ absolute requirement for a divalent cation, 87% of the activation with Mg2+ Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.52 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.52 partial Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.52 liver
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.52 0.003275
-
-
Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.1.52 -16°C, about 30% loss of activity within 24 h and about 60% loss of activity within 72 h Sus scrofa
2.7.1.52 -16°C, in the presence of stabilizing ovalbumin, about 30% loss of activity within 48 h Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.52 ATP + 6-deoxy-L-galactose
-
Sus scrofa ADP + 6-deoxy-L-galactose 1-phosphate beta-L-fucose 1-phosphate ?
2.7.1.52 ATP + D-arabinose 28% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 ATP + D-glucose 107% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 ATP + D-ribose 87% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 ATP + L-arabinose 31% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 ATP + L-rhamnose 41% of the activity with L-fucose Sus scrofa ?
-
?
2.7.1.52 CTP + 6-deoxy-L-galactose 60% of the activity with ATP Sus scrofa CDP + 6-deoxy-L-galactose 1-phosphate
-
?
2.7.1.52 GTP + 6-deoxy-L-galactose 56% of the activity with ATP Sus scrofa GDP + 6-deoxy-L-galactose 1-phosphate
-
?
2.7.1.52 additional information less than 5% of the activity with L-fucose is observed with: D-mannose, D-galactose, D-xylose, L-xylose, D-fucose and L-fucolose Sus scrofa ?
-
?
2.7.1.52 UTP + 6-deoxy-L-galactose 59% of the activity with ATP Sus scrofa UDP + 6-deoxy-L-galactose 1-phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.52 8
-
-
Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.52 7 9.5 pH 7: about 50% of maximal activity, pH 9.5: about 60% of maximal activity Sus scrofa