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Literature summary extracted from

  • Valentin-Hansen, P.
    Uridine-cytidine kinase from Escherichia coli (1978), Methods Enzymol., 51, 308-314.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.48 enzyme is unstable in crude extracts, especially in the presence of Mg2+ Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.48 CTP
-
 Escherichia coli
2.7.1.48 UTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.48 0.13
-
 cytidine pH 7.8, 37°C Escherichia coli
2.7.1.48 0.35
-
 uridine pH 7.8, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.48 90000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.48 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.48 ultracentrifugation, DEAE-cellulose, affinity chromatography on 5'amino-5'-deoxyuridine Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.48 200
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.1.48 4°C, 100 mM Tris-HCl, pH 7.8, 6 months, no loss of activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.48 ATP + cytidine
-
 Escherichia coli ADP + CMP
-
 ?
2.7.1.48 ATP + uridine dGTP and GTP are the most efficient phosphate donors, activity with ATP and dATP is 10% and with dUTP, dCTP and dTTP 5% of that with GTP or dGTP Escherichia coli ADP + UMP
-
 ?
2.7.1.48 dGTP + uridine
-
 Escherichia coli dGDP + UMP
-
 ?
2.7.1.48 GTP + 5-fluorocytidine 40% of activity with uridine Escherichia coli GDP + 5-fluorocytidine 5'-monophosphate
-
 ?
2.7.1.48 GTP + 5-fluorouridine 60% of activity with uridine Escherichia coli GDP + 5-fluorouridine 5'-monophosphate
-
 ?
2.7.1.48 GTP + 6-azauridine 31% of activity with uridine Escherichia coli GDP 6-azauridine 5'-monophosphate
-
 ?
2.7.1.48 GTP + cytidine
-
 Escherichia coli CMP + GDP
-
 ?
2.7.1.48 GTP + N-acetylcytidine 57% of activity with uridine Escherichia coli GDP + N-acetylcytidine 5'-monophosphate
-
 ?
2.7.1.48 GTP + uridine
-
 Escherichia coli UMP + GDP
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.48 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.48 7.8
-
 broad optimum Escherichia coli