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Literature summary extracted from

  • Plaxton, W.C.; Smith, C.R.; Knowles, V.L.
    Molecular and regulatory properties of leucoplast pyruvate kinase from Brassica napus (rapeseed) suspension cells (2002), Arch. Biochem. Biophys., 400, 54-62.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 6-phosphogluconate at 1 mM, 200% of activity Brassica napus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2-oxoglutarate 50% inhibition at 8.3 mM Brassica napus
2.7.1.40 citrate 50% inhibition at 18.4 mM Brassica napus
2.7.1.40 oxalate 50% inhibition at 0.23 mM Brassica napus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.052
-
 phosphoenolpyruvate 24°C, pH 8.0 Brassica napus
2.7.1.40 0.14
-
 ADP pH 8.0, 24°C Brassica napus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 58000
-
 3 * 58000 + 3 64000, SDS-PAGE Brassica napus
2.7.1.40 380000
-
 gel filtration Brassica napus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Brassica napus
-
 PKp, plastidic form
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
 Brassica napus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate
-
 Brassica napus ATP + pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 hexamer 3 * 58000 + 3 64000, SDS-PAGE Brassica napus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 8
-
-
 Brassica napus