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Literature summary extracted from

  • Knowles, V.L.; Smith, C.S.; Smith, C.R.; Plaxton, W.C.
    Structural and regulatory properties of pyruvate kinase from the cyanobacterium Synechococcus PCC 6301 (2001), J. Biol. Chem., 276, 20966-20972.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 AMP at 1 mM or below Synechococcus sp.
2.7.1.40 D-ribose 5-phosphate
-
Synechococcus sp.
2.7.1.40 glycerol 3-phosphate
-
Synechococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.40 2-oxoglutarate at 5 mM, pH 7.0, 35% inhibition Synechococcus sp.
2.7.1.40 ATP at 1 mM, pH 7.0, 50% inhibition Synechococcus sp.
2.7.1.40 citrate at 5 mM, pH 7.0, 40% inhibition Synechococcus sp.
2.7.1.40 fructose 1,6-diphosphate at 5 mM, pH 7.0, 60% inhibition Synechococcus sp.
2.7.1.40 phosphate at 5 mM, pH 7.0, 40% inhibition Synechococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.40 0.45
-
phosphoenolpyruvate allosteric enzyme, pH 7.5, 24°C Synechococcus sp.
2.7.1.40 0.54
-
phosphoenolpyruvate allosteric enzyme, pH 7.0, 24°C Synechococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.40 K+ not Synechococcus sp.
2.7.1.40 Mg2+ requirement Synechococcus sp.
2.7.1.40 Mg2+ either Mn2+ or Mg2+ are required Synechococcus sp.
2.7.1.40 Mn2+ either Mn2+ or Mg2+ are required Synechococcus sp.
2.7.1.40 Na+ not Synechococcus sp.
2.7.1.40 NH4+ not Synechococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.40 66000
-
4 * 66000, SDS-PAGE Synechococcus sp.
2.7.1.40 280000
-
gel filtration Synechococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Synechococcus sp.
-
cyanobacterium, strain PCC 6301
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.40
-
Synechococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.40 ADP + phosphoenolpyruvate ADP preferred substrate, UDP, IDP, GDP and CDP may also be used with lower effectivity Synechococcus sp. ATP + pyruvate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer 4 * 66000, SDS-PAGE Synechococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.40 7
-
-
Synechococcus sp.